Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora
Ano de defesa: | 2022 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
UFSM Frederico Westphalen |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Agronomia - Agricultura e Ambiente
|
Departamento: |
Agronomia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/27575 |
Resumo: | Soybean (Glycine max (L.) Merrill) plays a major role in the global commodity scenario. Regarding the biological agents that cause losses in soybean grain yield, there are phytophthora root rot, caused by Phytophthora sojae. One of the most efficient ways to control phytophthora root rot in soybean is genetic resistance. In front of this, this work aimed to characterize phenotypically genotypes carrying Rps genes, as well as the populations generated by the pyramiding of these genes, through the virulence differentiation obtained by inoculation of P. sojae pathotypes. The experiments were divided into stages and conducted in two locations. The phenotypic characterization of the parents carrying Rps genes was carried out in a field experiment in the municipality of Passo Fundo/RS in the 2020/2021 crop season, where phenotypic characteristics and grain yield were evaluated. In the municipality of Cambé/PR, in the years 2020 to 2022, the following steps were carried out in a greenhouse: i) inoculation methodology in seedlings and lateral branches of adult plants in 17 genotypes; ii) hybridizations aiming to combine Rps genes; iii) inoculation and characterization of F1 populations; iv) inoculation and characterization of F2 populations; v) characterization of F2 populations to Rps genes. The characterizations were performed by inoculating the PS2.4, PS14.4, PS34.1, PS36.1 and CMES1608 pathotypes. The results emphasize that inoculations in both seedlings and lateral branches of adult plants allowed genotype characterization for the different pathotypes. Genotypes characterization allowed to identify new possibilities of resistance sources to control P. sojae. Rps pyramiding gene showed to be a great genetic tool for the control of P. sojae. It was possible to identify genotypes reaction and their inoculated progenies with the main pathotypes that cause phytophthora root rot present in Brazilian soils. It was possible to highlight the main Rps genes and their combinations, serving as a basis for new studies and practical applications in soybean breeding programs. |
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2023-01-12T12:43:07Z2023-01-12T12:43:07Z2022-10-25http://repositorio.ufsm.br/handle/1/27575Soybean (Glycine max (L.) Merrill) plays a major role in the global commodity scenario. Regarding the biological agents that cause losses in soybean grain yield, there are phytophthora root rot, caused by Phytophthora sojae. One of the most efficient ways to control phytophthora root rot in soybean is genetic resistance. In front of this, this work aimed to characterize phenotypically genotypes carrying Rps genes, as well as the populations generated by the pyramiding of these genes, through the virulence differentiation obtained by inoculation of P. sojae pathotypes. The experiments were divided into stages and conducted in two locations. The phenotypic characterization of the parents carrying Rps genes was carried out in a field experiment in the municipality of Passo Fundo/RS in the 2020/2021 crop season, where phenotypic characteristics and grain yield were evaluated. In the municipality of Cambé/PR, in the years 2020 to 2022, the following steps were carried out in a greenhouse: i) inoculation methodology in seedlings and lateral branches of adult plants in 17 genotypes; ii) hybridizations aiming to combine Rps genes; iii) inoculation and characterization of F1 populations; iv) inoculation and characterization of F2 populations; v) characterization of F2 populations to Rps genes. The characterizations were performed by inoculating the PS2.4, PS14.4, PS34.1, PS36.1 and CMES1608 pathotypes. The results emphasize that inoculations in both seedlings and lateral branches of adult plants allowed genotype characterization for the different pathotypes. Genotypes characterization allowed to identify new possibilities of resistance sources to control P. sojae. Rps pyramiding gene showed to be a great genetic tool for the control of P. sojae. It was possible to identify genotypes reaction and their inoculated progenies with the main pathotypes that cause phytophthora root rot present in Brazilian soils. It was possible to highlight the main Rps genes and their combinations, serving as a basis for new studies and practical applications in soybean breeding programs.A cultura da soja (Glycine max. (L.) Merrill) exerce elevado protagonismo no cenário mundial de commodities. Sobre os agentes biológicos causadores de perdas na produtividade de grãos na cultura da soja, temos a podridão radicular de fitóftora, causada por Phytophthora sojae. Uma das formas mais eficientes para o controle da podridão radicular de fitóftora em soja é a resistência genética. Diante do exposto, este trabalho teve como objetivo caracterizar fenotipicamente genótipos portadores de genes Rps, assim como as populações geradas pela piramidação desses genes, através da diferenciação de virulência obtida pela inoculação de patótipos de P. sojae. A caracterização fenotípica dos parentais portadores de genes Rps foi realizada em experimento a campo no município de Passo Fundo/RS na safra 2020/2021, onde foram avaliadas características fenotípicas e produtividade de grãos. No município de Cambé/PR, nos anos 2020 a 2022, foram realizadas as seguintes etapas em casa de vegetação: i) metodologia de inoculação em plântulas e ramos laterais de plantas adultas em 17 genótipos; ii) hibridações visando combinar genes Rps; iii) inoculação e caracterização de populações F1; iv) inoculação e caraterização de populações F2; v) caraterização de populações F2 para presença de genes Rps. As caracterizações foram realizadas através da inoculação dos patótipos PS2.4, PS14.4, PS34.1, PS36.1 e CMES1608. Os resultados ressaltam que a inoculação tanto em plântulas quanto em ramos laterais de plantas adultas permitiu a caracterização dos genótipos para os diferentes patótipos, além disso, a inoculação em ramos laterais de plantas adultas permitiu a realização de diferentes testes ao mesmo tempo, podendo ser testado mais de um isolado na mesma planta. A caracterização dos genótipos permite identificar novas possibilidades de fontes de resistência para manejar P. sojae. A piramidação de genes Rps mostrou ser uma ótima ferramenta genética para controle de P. sojae. Foi possível identificar a reação de genótipos e de suas progênies inoculadas com os principais patótipos causadores de podridão radicular de fitóftora presentes em solos brasileiros. Foi possível destacar os principais genes Rps e suas combinações, servindo como base para novos estudos e aplicações práticas em programas de melhoramento genético de soja.porUniversidade Federal de Santa MariaUFSM Frederico WestphalenPrograma de Pós-Graduação em Agronomia - Agricultura e AmbienteUFSMBrasilAgronomiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPhytophthora sojaeGlycine max MerrilInoculação de patótiposPathotype inoculationCNPQ::CIENCIAS AGRARIAS::AGRONOMIACaracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftoraGenotypes soybean characterization and Rps genes pyramidation for phytophthora resistanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMarchioro, Volmir Sergiohttp://lattes.cnpq.br/3744130894870798Toebe, MarcosMeira, DanielaBenin, Giovanihttp://lattes.cnpq.br/9242686396364499Santos, Guilherme dos5001000000096006006006006006009455af8c-9ffa-465d-a5a2-0a472c7c4230b790280c-45a7-48aa-9ee1-a8a9c101bc39def97000-8854-4ff1-82fe-c38ea4c1c4643e17da55-58b2-4ea5-a470-20e73fee69a47a9217ac-4282-4df9-9b94-0676fb19f8fareponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/27575/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALDIS_PPGAAA_2022_SANTOS_GUILHERME.pdfDIS_PPGAAA_2022_SANTOS_GUILHERME.pdfDissertação de Mestradoapplication/pdf1993541http://repositorio.ufsm.br/bitstream/1/27575/1/DIS_PPGAAA_2022_SANTOS_GUILHERME.pdf783fcbf78089fef4e24f96b217c32565MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/27575/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/275752023-01-12 09:43:07.497oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-01-12T12:43:07Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
dc.title.alternative.eng.fl_str_mv |
Genotypes soybean characterization and Rps genes pyramidation for phytophthora resistance |
title |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
spellingShingle |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora Santos, Guilherme dos Phytophthora sojae Glycine max Merril Inoculação de patótipos Pathotype inoculation CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
title_full |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
title_fullStr |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
title_full_unstemmed |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
title_sort |
Caracterização de genótipos de soja e piramidação de genes Rps para resistência à fitóftora |
author |
Santos, Guilherme dos |
author_facet |
Santos, Guilherme dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Marchioro, Volmir Sergio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3744130894870798 |
dc.contributor.advisor-co1.fl_str_mv |
Toebe, Marcos |
dc.contributor.referee1.fl_str_mv |
Meira, Daniela |
dc.contributor.referee2.fl_str_mv |
Benin, Giovani |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9242686396364499 |
dc.contributor.author.fl_str_mv |
Santos, Guilherme dos |
contributor_str_mv |
Marchioro, Volmir Sergio Toebe, Marcos Meira, Daniela Benin, Giovani |
dc.subject.por.fl_str_mv |
Phytophthora sojae Glycine max Merril Inoculação de patótipos |
topic |
Phytophthora sojae Glycine max Merril Inoculação de patótipos Pathotype inoculation CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Pathotype inoculation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Soybean (Glycine max (L.) Merrill) plays a major role in the global commodity scenario. Regarding the biological agents that cause losses in soybean grain yield, there are phytophthora root rot, caused by Phytophthora sojae. One of the most efficient ways to control phytophthora root rot in soybean is genetic resistance. In front of this, this work aimed to characterize phenotypically genotypes carrying Rps genes, as well as the populations generated by the pyramiding of these genes, through the virulence differentiation obtained by inoculation of P. sojae pathotypes. The experiments were divided into stages and conducted in two locations. The phenotypic characterization of the parents carrying Rps genes was carried out in a field experiment in the municipality of Passo Fundo/RS in the 2020/2021 crop season, where phenotypic characteristics and grain yield were evaluated. In the municipality of Cambé/PR, in the years 2020 to 2022, the following steps were carried out in a greenhouse: i) inoculation methodology in seedlings and lateral branches of adult plants in 17 genotypes; ii) hybridizations aiming to combine Rps genes; iii) inoculation and characterization of F1 populations; iv) inoculation and characterization of F2 populations; v) characterization of F2 populations to Rps genes. The characterizations were performed by inoculating the PS2.4, PS14.4, PS34.1, PS36.1 and CMES1608 pathotypes. The results emphasize that inoculations in both seedlings and lateral branches of adult plants allowed genotype characterization for the different pathotypes. Genotypes characterization allowed to identify new possibilities of resistance sources to control P. sojae. Rps pyramiding gene showed to be a great genetic tool for the control of P. sojae. It was possible to identify genotypes reaction and their inoculated progenies with the main pathotypes that cause phytophthora root rot present in Brazilian soils. It was possible to highlight the main Rps genes and their combinations, serving as a basis for new studies and practical applications in soybean breeding programs. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-10-25 |
dc.date.accessioned.fl_str_mv |
2023-01-12T12:43:07Z |
dc.date.available.fl_str_mv |
2023-01-12T12:43:07Z |
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por |
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500100000009 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria UFSM Frederico Westphalen |
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Programa de Pós-Graduação em Agronomia - Agricultura e Ambiente |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Agronomia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria UFSM Frederico Westphalen |
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