Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/10036 |
Resumo: | The aim of this study was to evaluate the effect of fibroblast growth factor 10 (FGF10) on bovine oocyte meiotic resumption in an in vitro oocyte and follicular cells co-culture system. At first, an oil-free culture system able to maintain a stable osmolality of the medium without the negative interference of the oil was established. The maintenance of osmotic equilibrium confirms that fourwell plate with water in the central hole can be a feasible alternative to replace oil for scientific experimentation and embryo culture. The embryo development rate in the alternative system (near 30% blastocyst) was similar to that obtained in the conventional system (33% blastocyst). Afterwards, the role of FGF10 on bovine oocyte meiotic resumption was evaluated in a co-culture of oocytes and follicular cells in the presence of angiotensin II (10-11M AngII). All tested concentrations of FGF10 inhibited the resumption of meiosis induced by AngII (P ≤ 0.05). The highest germinal vesicle breakdown (GVBD) rate was observed when FGF10 was absent (45.3%). At concentrations of 10, 100, and 1000ng mL-1 FGF10, the rates of GVBD were 30.2, 29.6 and 27%, respectively. In a second experiment, oocytes were co-cultured with follicular hemisections to test if FGF10 (100ng mL-1) acts directly on COCs or through follicular cells to inhibit AngII (10-11M)-induced meiotic resumption. The AngII-induced GVBD (62.6%) was inhibited when FGF10 was added to in vitro co-culture system (37.8%; P ≤ 0.05). However, FGF10 did not affect meiotic resumption of COCs cultured without AngII in the presence or absence of follicular cells. The third experiment tested if FGF10 inhibits progesterone-induced meiotic resumption. The addition of FGF10 did not affect the meiotic resumption rate induced by progesterone (41.1% and 41.7% GVBD with and without FGF10, respectively; P ≥ 0.05). However, indomethacin (10μM) blocked (20.1% GVBD; P ≤ 0.05) progesterone positive effect suggesting that this steroid, like AngII, acts through cyclooxygenases pathway to induce meiotic resumption. Results show that FGF10 interacts with follicular cells to inhibit AngII but not progesterone-induced meiotic resumption of bovine oocytes. |
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2017-05-302017-05-302009-02-27GASPERIN, Bernardo Garziera. Modulation of meiotic resumption of bovine oocytes by fibroblast growth factor 10, angiotensin ii and progesterone in an alternative culture system. 2009. 72 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009.http://repositorio.ufsm.br/handle/1/10036The aim of this study was to evaluate the effect of fibroblast growth factor 10 (FGF10) on bovine oocyte meiotic resumption in an in vitro oocyte and follicular cells co-culture system. At first, an oil-free culture system able to maintain a stable osmolality of the medium without the negative interference of the oil was established. The maintenance of osmotic equilibrium confirms that fourwell plate with water in the central hole can be a feasible alternative to replace oil for scientific experimentation and embryo culture. The embryo development rate in the alternative system (near 30% blastocyst) was similar to that obtained in the conventional system (33% blastocyst). Afterwards, the role of FGF10 on bovine oocyte meiotic resumption was evaluated in a co-culture of oocytes and follicular cells in the presence of angiotensin II (10-11M AngII). All tested concentrations of FGF10 inhibited the resumption of meiosis induced by AngII (P ≤ 0.05). The highest germinal vesicle breakdown (GVBD) rate was observed when FGF10 was absent (45.3%). At concentrations of 10, 100, and 1000ng mL-1 FGF10, the rates of GVBD were 30.2, 29.6 and 27%, respectively. In a second experiment, oocytes were co-cultured with follicular hemisections to test if FGF10 (100ng mL-1) acts directly on COCs or through follicular cells to inhibit AngII (10-11M)-induced meiotic resumption. The AngII-induced GVBD (62.6%) was inhibited when FGF10 was added to in vitro co-culture system (37.8%; P ≤ 0.05). However, FGF10 did not affect meiotic resumption of COCs cultured without AngII in the presence or absence of follicular cells. The third experiment tested if FGF10 inhibits progesterone-induced meiotic resumption. The addition of FGF10 did not affect the meiotic resumption rate induced by progesterone (41.1% and 41.7% GVBD with and without FGF10, respectively; P ≥ 0.05). However, indomethacin (10μM) blocked (20.1% GVBD; P ≤ 0.05) progesterone positive effect suggesting that this steroid, like AngII, acts through cyclooxygenases pathway to induce meiotic resumption. Results show that FGF10 interacts with follicular cells to inhibit AngII but not progesterone-induced meiotic resumption of bovine oocytes.O presente trabalho teve por objetivo averiguar o papel do fator de crescimento fibroblástico 10 (FGF10) no reinício da meiose de oócitos bovinos, utilizando um modelo in vitro de co-cultivo de oócitos e células foliculares. Em um primeiro estudo, foi estabelecido um sistema de cultivo capaz de manter a osmolalidade dos meios constante na ausência de óleo. A adição de água entre os poços de placas de cultivo foi eficiente na prevenção da evaporação dos meios sendo uma alternativa na experimentação científica e produção in vitro de embriões. Com este sistema, se obteve uma taxa média de 30% de desenvolvimento embrionário, resultado similar ao obtido no sistema convencional (33% blastocistos). Após o estabelecimento do sistema, avaliou-se a participação do FGF10 no reinício da meiose de oócitos bovinos e sua interação com a maturação nuclear induzida por angiotensina II (AngII; 10-11M) e progesterona (100ng mL-1). Todas as concentrações de FGF10 testadas foram eficientes na inibição do reinício da meiose induzido por AngII (P ≤ 0,05). As maiores taxas de rompimento de vesícula germinativa (RVG) foram observadas na ausência de FGF10 (45,3%). Nas concentrações de 10, 100, e 1000ng mL-1 de FGF10, as taxas de RVG foram 30,2, 29,6 e 27%, respectivamente. Após o estabelecimento da dose ideal, foi verificado que a adição de FGF10 (100ng mL-1) ao cultivo de complexos cumulus-oócitos acarretou em diminuição na taxa de reinício da meiose somente na presença de células foliculares e AngII (37,8% e 62,6% de RVG com e sem FGF10, respectivamente; P ≤ 0,05). A partir dos resultados dos experimentos anteriores, o terceiro experimento foi delineado para testar se o FGF10 é capaz de inibir o reinício da divisão meiótica induzido por progesterona. A adição de FGF10 não afetou a taxa de reinício da meiose induzida pela progesterona (41,1% e 41,7% de RVG com e sem FGF10, respectivamente; P ≥ 0,05). Entretanto, a adição de indometacina (10μM) bloqueou (20,1% de RVG; P ≤ 0,05) o efeito positivo da progesterona. Este bloqueio demonstra que a progesterona, assim como já foi demonstrado para AngII, utiliza a via das ciclooxigenases para induzir o reinício da meiose e este evento parece não ser regulado pelo FGF10. Os resultados demonstram que o FGF10 interage com as células foliculares para inibir o reinício da meiose estimulado pela AngII mas não pela progesterona.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaFGF10Angiotensina IIProgesteronaReinício da meioseOócitos bovinosAngiotensin IIProgesteroneMeiotic resumptionBovine oocytesCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAModulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivoModulation of meiotic resumption of bovine oocytes by fibroblast growth factor 10, angiotensin II and progesterone in an alternative culture systeminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisGonçalves, Paulo Bayard Diashttp://lattes.cnpq.br/5837260966665885Oliveira, João Francisco Coelho dehttp://lattes.cnpq.br/1168979725730267Costa, Luís Fabiano Santos dahttp://lattes.cnpq.br/0382799433347639Pegoraro, Lígia Margareth Cantarellihttp://lattes.cnpq.br/1286067347305118http://lattes.cnpq.br/2925436755759308Gasperin, Bernardo Garziera50050000000740050050030030030039e380be-dcb6-45dc-96d0-ad18ba64f2b3030d815b-50d4-446b-9ff4-cea7cd8987f1b53a8c5b-a39e-469a-a6e2-57c969fd72c7579a65db-d8f0-4fe9-8b4d-82a3fcbbc94bbe769fcc-55ec-459e-8c36-3884beaf2e28info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBERNARDOGARZIERAGASPERIN.pdfDissertação de Mestradoapplication/pdf1055395http://repositorio.ufsm.br/bitstream/1/10036/1/BERNARDOGARZIERAGASPERIN.pdfc3d5901e2b9e3d4d40ad8fdc0c12de12MD51TEXTBERNARDOGARZIERAGASPERIN.pdf.txtBERNARDOGARZIERAGASPERIN.pdf.txtExtracted texttext/plain138878http://repositorio.ufsm.br/bitstream/1/10036/2/BERNARDOGARZIERAGASPERIN.pdf.txtf5e200ea3256d87bec215ff588d1109dMD52THUMBNAILBERNARDOGARZIERAGASPERIN.pdf.jpgBERNARDOGARZIERAGASPERIN.pdf.jpgIM Thumbnailimage/jpeg5501http://repositorio.ufsm.br/bitstream/1/10036/3/BERNARDOGARZIERAGASPERIN.pdf.jpg8a89c1049baccbb60941d6574b8c5b60MD531/100362023-04-27 15:29:15.057oai:repositorio.ufsm.br:1/10036Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-04-27T18:29:15Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
dc.title.alternative.eng.fl_str_mv |
Modulation of meiotic resumption of bovine oocytes by fibroblast growth factor 10, angiotensin II and progesterone in an alternative culture system |
title |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
spellingShingle |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo Gasperin, Bernardo Garziera FGF10 Angiotensina II Progesterona Reinício da meiose Oócitos bovinos Angiotensin II Progesterone Meiotic resumption Bovine oocytes CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
title_full |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
title_fullStr |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
title_full_unstemmed |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
title_sort |
Modulação do reinício da meiose de oócitos bovinos pelo fator de crescimento fibroblástico 10, angiotensina II e progesterona em sistema alternativo de cultivo |
author |
Gasperin, Bernardo Garziera |
author_facet |
Gasperin, Bernardo Garziera |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Gonçalves, Paulo Bayard Dias |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5837260966665885 |
dc.contributor.advisor-co1.fl_str_mv |
Oliveira, João Francisco Coelho de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1168979725730267 |
dc.contributor.referee1.fl_str_mv |
Costa, Luís Fabiano Santos da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0382799433347639 |
dc.contributor.referee2.fl_str_mv |
Pegoraro, Lígia Margareth Cantarelli |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1286067347305118 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2925436755759308 |
dc.contributor.author.fl_str_mv |
Gasperin, Bernardo Garziera |
contributor_str_mv |
Gonçalves, Paulo Bayard Dias Oliveira, João Francisco Coelho de Costa, Luís Fabiano Santos da Pegoraro, Lígia Margareth Cantarelli |
dc.subject.por.fl_str_mv |
FGF10 Angiotensina II Progesterona Reinício da meiose Oócitos bovinos |
topic |
FGF10 Angiotensina II Progesterona Reinício da meiose Oócitos bovinos Angiotensin II Progesterone Meiotic resumption Bovine oocytes CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Angiotensin II Progesterone Meiotic resumption Bovine oocytes |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The aim of this study was to evaluate the effect of fibroblast growth factor 10 (FGF10) on bovine oocyte meiotic resumption in an in vitro oocyte and follicular cells co-culture system. At first, an oil-free culture system able to maintain a stable osmolality of the medium without the negative interference of the oil was established. The maintenance of osmotic equilibrium confirms that fourwell plate with water in the central hole can be a feasible alternative to replace oil for scientific experimentation and embryo culture. The embryo development rate in the alternative system (near 30% blastocyst) was similar to that obtained in the conventional system (33% blastocyst). Afterwards, the role of FGF10 on bovine oocyte meiotic resumption was evaluated in a co-culture of oocytes and follicular cells in the presence of angiotensin II (10-11M AngII). All tested concentrations of FGF10 inhibited the resumption of meiosis induced by AngII (P ≤ 0.05). The highest germinal vesicle breakdown (GVBD) rate was observed when FGF10 was absent (45.3%). At concentrations of 10, 100, and 1000ng mL-1 FGF10, the rates of GVBD were 30.2, 29.6 and 27%, respectively. In a second experiment, oocytes were co-cultured with follicular hemisections to test if FGF10 (100ng mL-1) acts directly on COCs or through follicular cells to inhibit AngII (10-11M)-induced meiotic resumption. The AngII-induced GVBD (62.6%) was inhibited when FGF10 was added to in vitro co-culture system (37.8%; P ≤ 0.05). However, FGF10 did not affect meiotic resumption of COCs cultured without AngII in the presence or absence of follicular cells. The third experiment tested if FGF10 inhibits progesterone-induced meiotic resumption. The addition of FGF10 did not affect the meiotic resumption rate induced by progesterone (41.1% and 41.7% GVBD with and without FGF10, respectively; P ≥ 0.05). However, indomethacin (10μM) blocked (20.1% GVBD; P ≤ 0.05) progesterone positive effect suggesting that this steroid, like AngII, acts through cyclooxygenases pathway to induce meiotic resumption. Results show that FGF10 interacts with follicular cells to inhibit AngII but not progesterone-induced meiotic resumption of bovine oocytes. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-02-27 |
dc.date.accessioned.fl_str_mv |
2017-05-30 |
dc.date.available.fl_str_mv |
2017-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GASPERIN, Bernardo Garziera. Modulation of meiotic resumption of bovine oocytes by fibroblast growth factor 10, angiotensin ii and progesterone in an alternative culture system. 2009. 72 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10036 |
identifier_str_mv |
GASPERIN, Bernardo Garziera. Modulation of meiotic resumption of bovine oocytes by fibroblast growth factor 10, angiotensin ii and progesterone in an alternative culture system. 2009. 72 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
url |
http://repositorio.ufsm.br/handle/1/10036 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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500500000007 |
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400 500 500 300 300 300 |
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openAccess |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Medicina Veterinária |
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UFSM |
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BR |
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Medicina Veterinária |
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Universidade Federal de Santa Maria |
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