Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
| Ano de defesa: | 2006 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Dissertação |
| Tipo de acesso: | Acesso aberto |
| dARK ID: | ark:/26339/00130000034xv |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal de Santa Maria
BR Zootecnia UFSM Programa de Pós-Graduação em Zootecnia |
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Palavras-chave em Português: | |
| Link de acesso: | http://repositorio.ufsm.br/handle/1/10801 |
Resumo: | The aim of this study was to determine the mean lethal concentration (96 h) for waterborne copper (LC50), as well as the effect of the exposure to copper on growth, metabolic parameters (glycogen, glucose, lactate, and protein) in some tissues, activity of acetylcholinesterase (AChE) (brain and muscle), amylase and maltase (intestine) in silver catfish (Rhamdia quelen). The LC50 for copper was 0.4 mg/L. On growth experiments fish were exposed to 10 and 20% LC50, i.e., 0.04 and 0.08 mg/L respectively. Exposure to copper did not change growth parameters (weight, length and biomass). In the liver, lactate levels increased in juveniles exposed to 0.04 mg/L and decreased in those maintained at 0.08 mg/L, while protein levels decreased in those exposed to both concentrations compared to unexposed specimens. Glycogen levels in the muscle were lower in fish exposed to both concentrations, glucose and lactate were higher in those exposed to 0.04 mg/L and decreased in juveniles maintained at 0.08 mg/L, while protein was higher in those exposed to 0.08 mg/L. Glucose and lactate plasma levels were higher in juveniles exposed to 0.04 mg/L, but protein levels were lower in those maintained at both copper concentrations. Amylase activity was lower in juveniles exposed to both concentrations, but maltase was higher in those exposed to 0.04 mg/L than control group. Brain AChE activity was lower in fish exposed to both concentrations while muscle AChE activity was not affected after 45 days of exposure. It can be concluded that copper can change several metabolic parameters and enzymes of toxicological and feeding interest even at sublethal concentrations. |
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Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobreSurvival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copperPsiculturaJundiáEnzimasCobreLethal concentrationGrowthMetabolic parametersAcetylcholinesteraseAmylaseMaltaseSilver catfish (Rhamdia quelen.)CNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThe aim of this study was to determine the mean lethal concentration (96 h) for waterborne copper (LC50), as well as the effect of the exposure to copper on growth, metabolic parameters (glycogen, glucose, lactate, and protein) in some tissues, activity of acetylcholinesterase (AChE) (brain and muscle), amylase and maltase (intestine) in silver catfish (Rhamdia quelen). The LC50 for copper was 0.4 mg/L. On growth experiments fish were exposed to 10 and 20% LC50, i.e., 0.04 and 0.08 mg/L respectively. Exposure to copper did not change growth parameters (weight, length and biomass). In the liver, lactate levels increased in juveniles exposed to 0.04 mg/L and decreased in those maintained at 0.08 mg/L, while protein levels decreased in those exposed to both concentrations compared to unexposed specimens. Glycogen levels in the muscle were lower in fish exposed to both concentrations, glucose and lactate were higher in those exposed to 0.04 mg/L and decreased in juveniles maintained at 0.08 mg/L, while protein was higher in those exposed to 0.08 mg/L. Glucose and lactate plasma levels were higher in juveniles exposed to 0.04 mg/L, but protein levels were lower in those maintained at both copper concentrations. Amylase activity was lower in juveniles exposed to both concentrations, but maltase was higher in those exposed to 0.04 mg/L than control group. Brain AChE activity was lower in fish exposed to both concentrations while muscle AChE activity was not affected after 45 days of exposure. It can be concluded that copper can change several metabolic parameters and enzymes of toxicological and feeding interest even at sublethal concentrations.O objetivo deste estudo foi verificar a concentração letal média em 96 h (CL50) para o cobre, bem como o efeito da exposição ao cobre sobre o crescimento, parâmetros metabólicos (glicogênio, glicose, lactato e proteína) em alguns tecidos (fígado, músculo e cérebro) e a atividade da acetilcolinesterase (AChE) (cérebro e músculo), amilase e maltase (intestino) em jundiás (Rhamdia quelen.). A CL50 para o cobre foi 0,4 mg/L. Nos experimentos de crescimento os peixes foram expostos durante 45 dias a 10 e 20% da CL50, ou seja, 0,04 e 0,08 mg/L respectivamente. A adição de cobre não alterou os parâmetros de crescimento avaliados (peso, comprimento e biomassa). No fígado, os níveis de lactato aumentaram nos exemplares expostos a 0,04 mg/L e diminuíram nos mantidos em 0,08 mg/L, enquanto que os níveis de proteína diminuíram em ambas as concentrações em relação ao grupo controle. No músculo houve redução na atividade do glicogênio nos exemplares mantidos nas duas concentrações testadas, a glicose e o lactato aumentaram nos expostos a 0,04 mg/L e diminuíram nos expostos a 0,08 mg/L, e a proteína aumentou nos mantidos em 0,08 mg/L. Os níveis de glicose e lactato no plasma foram maiores nos exemplares mantidos em 0,04 mg/L e diminuíram os níveis de proteína nos expostos a ambas as concentrações de cobre. A atividade da amilase foi menor nos juvenis expostos a ambas as concentrações, enquanto a da maltase foi maior em 0,04 mg/L quando comparada ao grupo controle. A atividade da AChE cerebral foi menor nos exemplares expostos a ambas concentrações, enquanto que a AChE muscular não sofreu alterações após os 45 dias de exposição. Conclui-se que o cobre mesmo em concentrações subletais pode alterar diversos parâmetros metabólicos e enzimas de interesse toxicológico e alimentar.Universidade Federal de Santa MariaBRZootecniaUFSMPrograma de Pós-Graduação em ZootecniaBaldisserotto, Bernardohttp://lattes.cnpq.br/1036046601275319Barcellos, Leonardo José Gilhttp://lattes.cnpq.br/4504482634911546Pavanato, Maria Amáliahttp://lattes.cnpq.br/8701892865724171Silva, Vera Maria Machado da2007-02-272007-02-272006-12-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSILVA, Vera Maria Machado da. Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper. 2006. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2006.http://repositorio.ufsm.br/handle/1/10801ark:/26339/00130000034xvporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-02-02T18:05:33Zoai:repositorio.ufsm.br:1/10801Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/PUBhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.bropendoar:2023-02-02T18:05:33Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
| dc.title.none.fl_str_mv |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper |
| title |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre |
| spellingShingle |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre Silva, Vera Maria Machado da Psicultura Jundiá Enzimas Cobre Lethal concentration Growth Metabolic parameters Acetylcholinesterase Amylase Maltase Silver catfish (Rhamdia quelen.) CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
| title_short |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre |
| title_full |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre |
| title_fullStr |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre |
| title_full_unstemmed |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre |
| title_sort |
Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre |
| author |
Silva, Vera Maria Machado da |
| author_facet |
Silva, Vera Maria Machado da |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
Baldisserotto, Bernardo http://lattes.cnpq.br/1036046601275319 Barcellos, Leonardo José Gil http://lattes.cnpq.br/4504482634911546 Pavanato, Maria Amália http://lattes.cnpq.br/8701892865724171 |
| dc.contributor.author.fl_str_mv |
Silva, Vera Maria Machado da |
| dc.subject.por.fl_str_mv |
Psicultura Jundiá Enzimas Cobre Lethal concentration Growth Metabolic parameters Acetylcholinesterase Amylase Maltase Silver catfish (Rhamdia quelen.) CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
| topic |
Psicultura Jundiá Enzimas Cobre Lethal concentration Growth Metabolic parameters Acetylcholinesterase Amylase Maltase Silver catfish (Rhamdia quelen.) CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
| description |
The aim of this study was to determine the mean lethal concentration (96 h) for waterborne copper (LC50), as well as the effect of the exposure to copper on growth, metabolic parameters (glycogen, glucose, lactate, and protein) in some tissues, activity of acetylcholinesterase (AChE) (brain and muscle), amylase and maltase (intestine) in silver catfish (Rhamdia quelen). The LC50 for copper was 0.4 mg/L. On growth experiments fish were exposed to 10 and 20% LC50, i.e., 0.04 and 0.08 mg/L respectively. Exposure to copper did not change growth parameters (weight, length and biomass). In the liver, lactate levels increased in juveniles exposed to 0.04 mg/L and decreased in those maintained at 0.08 mg/L, while protein levels decreased in those exposed to both concentrations compared to unexposed specimens. Glycogen levels in the muscle were lower in fish exposed to both concentrations, glucose and lactate were higher in those exposed to 0.04 mg/L and decreased in juveniles maintained at 0.08 mg/L, while protein was higher in those exposed to 0.08 mg/L. Glucose and lactate plasma levels were higher in juveniles exposed to 0.04 mg/L, but protein levels were lower in those maintained at both copper concentrations. Amylase activity was lower in juveniles exposed to both concentrations, but maltase was higher in those exposed to 0.04 mg/L than control group. Brain AChE activity was lower in fish exposed to both concentrations while muscle AChE activity was not affected after 45 days of exposure. It can be concluded that copper can change several metabolic parameters and enzymes of toxicological and feeding interest even at sublethal concentrations. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-12-13 2007-02-27 2007-02-27 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
SILVA, Vera Maria Machado da. Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper. 2006. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2006. http://repositorio.ufsm.br/handle/1/10801 |
| dc.identifier.dark.fl_str_mv |
ark:/26339/00130000034xv |
| identifier_str_mv |
SILVA, Vera Maria Machado da. Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper. 2006. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2006. ark:/26339/00130000034xv |
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http://repositorio.ufsm.br/handle/1/10801 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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Universidade Federal de Santa Maria BR Zootecnia UFSM Programa de Pós-Graduação em Zootecnia |
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Universidade Federal de Santa Maria BR Zootecnia UFSM Programa de Pós-Graduação em Zootecnia |
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reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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Manancial - Repositório Digital da UFSM |
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Manancial - Repositório Digital da UFSM |
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Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
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1847153338840252416 |