Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre

Detalhes bibliográficos
Ano de defesa: 2006
Autor(a) principal: Silva, Vera Maria Machado da
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
dARK ID: ark:/26339/00130000034xv
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
BR
Zootecnia
UFSM
Programa de Pós-Graduação em Zootecnia
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.ufsm.br/handle/1/10801
Resumo: The aim of this study was to determine the mean lethal concentration (96 h) for waterborne copper (LC50), as well as the effect of the exposure to copper on growth, metabolic parameters (glycogen, glucose, lactate, and protein) in some tissues, activity of acetylcholinesterase (AChE) (brain and muscle), amylase and maltase (intestine) in silver catfish (Rhamdia quelen). The LC50 for copper was 0.4 mg/L. On growth experiments fish were exposed to 10 and 20% LC50, i.e., 0.04 and 0.08 mg/L respectively. Exposure to copper did not change growth parameters (weight, length and biomass). In the liver, lactate levels increased in juveniles exposed to 0.04 mg/L and decreased in those maintained at 0.08 mg/L, while protein levels decreased in those exposed to both concentrations compared to unexposed specimens. Glycogen levels in the muscle were lower in fish exposed to both concentrations, glucose and lactate were higher in those exposed to 0.04 mg/L and decreased in juveniles maintained at 0.08 mg/L, while protein was higher in those exposed to 0.08 mg/L. Glucose and lactate plasma levels were higher in juveniles exposed to 0.04 mg/L, but protein levels were lower in those maintained at both copper concentrations. Amylase activity was lower in juveniles exposed to both concentrations, but maltase was higher in those exposed to 0.04 mg/L than control group. Brain AChE activity was lower in fish exposed to both concentrations while muscle AChE activity was not affected after 45 days of exposure. It can be concluded that copper can change several metabolic parameters and enzymes of toxicological and feeding interest even at sublethal concentrations.
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spelling Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobreSurvival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copperPsiculturaJundiáEnzimasCobreLethal concentrationGrowthMetabolic parametersAcetylcholinesteraseAmylaseMaltaseSilver catfish (Rhamdia quelen.)CNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThe aim of this study was to determine the mean lethal concentration (96 h) for waterborne copper (LC50), as well as the effect of the exposure to copper on growth, metabolic parameters (glycogen, glucose, lactate, and protein) in some tissues, activity of acetylcholinesterase (AChE) (brain and muscle), amylase and maltase (intestine) in silver catfish (Rhamdia quelen). The LC50 for copper was 0.4 mg/L. On growth experiments fish were exposed to 10 and 20% LC50, i.e., 0.04 and 0.08 mg/L respectively. Exposure to copper did not change growth parameters (weight, length and biomass). In the liver, lactate levels increased in juveniles exposed to 0.04 mg/L and decreased in those maintained at 0.08 mg/L, while protein levels decreased in those exposed to both concentrations compared to unexposed specimens. Glycogen levels in the muscle were lower in fish exposed to both concentrations, glucose and lactate were higher in those exposed to 0.04 mg/L and decreased in juveniles maintained at 0.08 mg/L, while protein was higher in those exposed to 0.08 mg/L. Glucose and lactate plasma levels were higher in juveniles exposed to 0.04 mg/L, but protein levels were lower in those maintained at both copper concentrations. Amylase activity was lower in juveniles exposed to both concentrations, but maltase was higher in those exposed to 0.04 mg/L than control group. Brain AChE activity was lower in fish exposed to both concentrations while muscle AChE activity was not affected after 45 days of exposure. It can be concluded that copper can change several metabolic parameters and enzymes of toxicological and feeding interest even at sublethal concentrations.O objetivo deste estudo foi verificar a concentração letal média em 96 h (CL50) para o cobre, bem como o efeito da exposição ao cobre sobre o crescimento, parâmetros metabólicos (glicogênio, glicose, lactato e proteína) em alguns tecidos (fígado, músculo e cérebro) e a atividade da acetilcolinesterase (AChE) (cérebro e músculo), amilase e maltase (intestino) em jundiás (Rhamdia quelen.). A CL50 para o cobre foi 0,4 mg/L. Nos experimentos de crescimento os peixes foram expostos durante 45 dias a 10 e 20% da CL50, ou seja, 0,04 e 0,08 mg/L respectivamente. A adição de cobre não alterou os parâmetros de crescimento avaliados (peso, comprimento e biomassa). No fígado, os níveis de lactato aumentaram nos exemplares expostos a 0,04 mg/L e diminuíram nos mantidos em 0,08 mg/L, enquanto que os níveis de proteína diminuíram em ambas as concentrações em relação ao grupo controle. No músculo houve redução na atividade do glicogênio nos exemplares mantidos nas duas concentrações testadas, a glicose e o lactato aumentaram nos expostos a 0,04 mg/L e diminuíram nos expostos a 0,08 mg/L, e a proteína aumentou nos mantidos em 0,08 mg/L. Os níveis de glicose e lactato no plasma foram maiores nos exemplares mantidos em 0,04 mg/L e diminuíram os níveis de proteína nos expostos a ambas as concentrações de cobre. A atividade da amilase foi menor nos juvenis expostos a ambas as concentrações, enquanto a da maltase foi maior em 0,04 mg/L quando comparada ao grupo controle. A atividade da AChE cerebral foi menor nos exemplares expostos a ambas concentrações, enquanto que a AChE muscular não sofreu alterações após os 45 dias de exposição. Conclui-se que o cobre mesmo em concentrações subletais pode alterar diversos parâmetros metabólicos e enzimas de interesse toxicológico e alimentar.Universidade Federal de Santa MariaBRZootecniaUFSMPrograma de Pós-Graduação em ZootecniaBaldisserotto, Bernardohttp://lattes.cnpq.br/1036046601275319Barcellos, Leonardo José Gilhttp://lattes.cnpq.br/4504482634911546Pavanato, Maria Amáliahttp://lattes.cnpq.br/8701892865724171Silva, Vera Maria Machado da2007-02-272007-02-272006-12-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSILVA, Vera Maria Machado da. Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper. 2006. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2006.http://repositorio.ufsm.br/handle/1/10801ark:/26339/00130000034xvporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-02-02T18:05:33Zoai:repositorio.ufsm.br:1/10801Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/PUBhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.bropendoar:2023-02-02T18:05:33Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper
title Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
spellingShingle Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
Silva, Vera Maria Machado da
Psicultura
Jundiá
Enzimas
Cobre
Lethal concentration
Growth
Metabolic parameters
Acetylcholinesterase
Amylase
Maltase
Silver catfish (Rhamdia quelen.)
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
title_short Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
title_full Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
title_fullStr Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
title_full_unstemmed Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
title_sort Sobrevivência, crescimento, parâmetros metabólicos e enzimáticos em jundiás (Rhamdia quelen) expostos ao cobre
author Silva, Vera Maria Machado da
author_facet Silva, Vera Maria Machado da
author_role author
dc.contributor.none.fl_str_mv Baldisserotto, Bernardo
http://lattes.cnpq.br/1036046601275319
Barcellos, Leonardo José Gil
http://lattes.cnpq.br/4504482634911546
Pavanato, Maria Amália
http://lattes.cnpq.br/8701892865724171
dc.contributor.author.fl_str_mv Silva, Vera Maria Machado da
dc.subject.por.fl_str_mv Psicultura
Jundiá
Enzimas
Cobre
Lethal concentration
Growth
Metabolic parameters
Acetylcholinesterase
Amylase
Maltase
Silver catfish (Rhamdia quelen.)
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
topic Psicultura
Jundiá
Enzimas
Cobre
Lethal concentration
Growth
Metabolic parameters
Acetylcholinesterase
Amylase
Maltase
Silver catfish (Rhamdia quelen.)
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
description The aim of this study was to determine the mean lethal concentration (96 h) for waterborne copper (LC50), as well as the effect of the exposure to copper on growth, metabolic parameters (glycogen, glucose, lactate, and protein) in some tissues, activity of acetylcholinesterase (AChE) (brain and muscle), amylase and maltase (intestine) in silver catfish (Rhamdia quelen). The LC50 for copper was 0.4 mg/L. On growth experiments fish were exposed to 10 and 20% LC50, i.e., 0.04 and 0.08 mg/L respectively. Exposure to copper did not change growth parameters (weight, length and biomass). In the liver, lactate levels increased in juveniles exposed to 0.04 mg/L and decreased in those maintained at 0.08 mg/L, while protein levels decreased in those exposed to both concentrations compared to unexposed specimens. Glycogen levels in the muscle were lower in fish exposed to both concentrations, glucose and lactate were higher in those exposed to 0.04 mg/L and decreased in juveniles maintained at 0.08 mg/L, while protein was higher in those exposed to 0.08 mg/L. Glucose and lactate plasma levels were higher in juveniles exposed to 0.04 mg/L, but protein levels were lower in those maintained at both copper concentrations. Amylase activity was lower in juveniles exposed to both concentrations, but maltase was higher in those exposed to 0.04 mg/L than control group. Brain AChE activity was lower in fish exposed to both concentrations while muscle AChE activity was not affected after 45 days of exposure. It can be concluded that copper can change several metabolic parameters and enzymes of toxicological and feeding interest even at sublethal concentrations.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-13
2007-02-27
2007-02-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Vera Maria Machado da. Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper. 2006. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2006.
http://repositorio.ufsm.br/handle/1/10801
dc.identifier.dark.fl_str_mv ark:/26339/00130000034xv
identifier_str_mv SILVA, Vera Maria Machado da. Survival, growth, metabolic and enzymatic parameters in silver catfish (Rhamdia quelen) exposed to waterborne copper. 2006. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2006.
ark:/26339/00130000034xv
url http://repositorio.ufsm.br/handle/1/10801
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Zootecnia
UFSM
Programa de Pós-Graduação em Zootecnia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Zootecnia
UFSM
Programa de Pós-Graduação em Zootecnia
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.br
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