Avaliação do perfil proteômico na saliva em trabalhadores da cadeia produtiva de joias, semijoias e bijuterias de Limeira, SP

• Ano de defesa: 2019
• Autor(a) principal: Araujo, Alda Neis Miranda De [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/48912/001300002htm0
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Proteomics; Post-Translational Modifications; Interatoma; Toxic Elements; Saliva; Informal Work; Proteômica; Modificações Pós-Traducionais; Elementos Tóxicos; Trabalho Informal
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7665245; https://repositorio.unifesp.br/handle/11600/59325

City of Limeira, located in Brazil, consolidates a region of jewelry and fashion adornment production with homemade and informal processes of assembly, welding and electroplating of jewels, providing a scenario of outsourced services. These processes can expose the worker to potentially toxic elements, such as mercury (Hg), lead (Pb), nickel (Ni), cadmium (Cd), zinc (Zn), arsenic (As), cupper (Cu), chrome (Cr) and tin (Sn). This study aimed to evaluate the post-translational modifications and the negative effects of metals on protein expression performing a proteomic approach on workers from the jewelry sector of Limeira city. Total saliva was collected from 26 participants, classified as Welders Group (n = 13) and Control Group (n = 13). The samples were analyzed using a nanoACQUITY UPLC® System coupled to a mass spectrometry system (maXis ™ 3G Q-TOF), in single shotgun LC-MS/MS runs. All the samples were analyzed using PEAKS Studio 8.5 software. The proteins identified in both groups were classified according to Gene Ontology (GO). Pathway enrichment analysis was performed using the Reactome Pathway Database, with a p-value ≤ 0.05 and a false discovery rate (FDR) ≤ 0.05 as the cut-off criteria. In bioinformatics analysis, 997 proteins were identified in the both groups: 119 proteins were expressed only in the welder group and others 136 proteins were expressed only in the control group. Principal component analysis, K-means clustering and dendogram construction were performed using Statistica 12.5 software. In the analysis of Gene Ontology the results revealed that the immune system was the second most represented GO process in the Controls group, being sub-categorized as immuno-effector, immune response, leukocyte activation and activation of the immune resistance system. These results indicated that welders may exhibit immunity deficiency. The welders were overrepresented in terms of metabolic process, catalytic activity, response to stimuli, response to toxic substances, metabolism activity, apoptosis, alteration in the cell cycle, stress response, terms (it’s) that suggest changes in protein expression associated with toxic effect of metals, which welders are exposed during their work life activities. The informal, home and outsourced works carried out by welders is influencing their internal exposure and this type of occupational activity should be considered to investigate associations with diseases and illness, those proteins that have been expressed only in the affected group.