Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae)
Ano de defesa: | 2007 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Viçosa
|
Programa de Pós-Graduação: |
Mestrado em Entomologia
|
Departamento: |
Ciência entomológica; Tecnologia entomológica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://locus.ufv.br/handle/123456789/3870 |
Resumo: | Protease inhibitors can reduce the digestive efficiency, to delay the development and to diminish the insect proteolytic activity resulting in increase of mortality through deficiency of free amino acids for maintenance of vital functions. The subject of this work were to evaluate if A. gemmatalis larvae feeding in artificial diets containing increasing concentrations of synthetic trypsin inhibitor bisbenzamidine affects negatively the insect performance, reduce the alimentary consumption, the digestibility, the assimilation efficiency of the food ingested and digested, the insect proteolytic activity and protein digestibility. To test these hypotheses were realized bioassays with individualized caterpillars and supplied daily with diets containing 0; 0,00095; 0,0019; 0,0038; 0,0076; 0,0152; 0,0304; 0,0608; 0,0912; 0,125 e 0,25% (w/w) of berenil during the entire larval phase. The digestion indices, the duration of larval phase and life cycle, the accumulated survival and the average of time survival, the pupae and adults weight, the body and wings of the adults moths were determined. Additionally, were evaluated the protein digestibility and the enzymatic activity of midgut extracts of A. gemmatalis exposed to berenil in diet, in the same concentrations above. All parameters assessed of life history were negatively affected with the increase of inhibitors in diet. Were verified an increase in the larval phase and life cycle duration, reduction in the pupae weight and body and wings length. There was reduction in the incorporated biomass and the relative growth rate. Otherwise, there were increases in ingestion, approximated digestion, and relative diet consumption rate and in the ingestion and excretion balance. The diet individual consumption increased until the 0.0076% of berenil, diminished with the increase of inhibitor concentration. The protein digestibility was directly proportional to berenil concentration while the proteolytic, amidolytic and esterolytic activities diminished with the increasing of inhibitor concentration. Therefore, albeit the berenil mixed in increasing concentrations in diet affects negatively in all life history parameters of insect, the caterpillars improve the diet intake. However, even so the total digestibility and the protein digestibility increase in higher inhibitor concentrations, the insect show lower enzymatic activity and, consequently, reduction in the efficiency in assimilation of ingested and digested food, indicating that the insect did not show adaptative mechanisms to prevent the inhibition of tripsina-like enzymes existents in your digestive tract. Thus, the berenil is a promising strategy to new studies with the subject of to synthesis mimicry peptides to apply in the field to control A. gemmatalis caterpillars in the soybean crops. |
id |
UFV_3796c25a46b7c9d4d783f30bb9340ede |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/3870 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
|
spelling |
Moreira, Lílian Fernandeshttp://lattes.cnpq.br/7479025604827544Campos, Wellington Garciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793941J6Oliveira, Joel Antônio dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707224A0Oliveira, Maria Goreti de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790894D6Andrade, Milton Hércules Guerra dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788176U9Guia, Thiago Rennó dos Mareshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763600P22015-03-26T13:30:20Z2009-02-162015-03-26T13:30:20Z2007-07-28MOREIRA, Lílian Fernandes. Effect of serine-proteases inhibitor, berenil, on the feeding efficiency, proteolytic activity and post- embryonic development of Anticarsia gemmatalis (Lepidoptera: Noctuidae). 2007. 123 f. Dissertação (Mestrado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2007.http://locus.ufv.br/handle/123456789/3870Protease inhibitors can reduce the digestive efficiency, to delay the development and to diminish the insect proteolytic activity resulting in increase of mortality through deficiency of free amino acids for maintenance of vital functions. The subject of this work were to evaluate if A. gemmatalis larvae feeding in artificial diets containing increasing concentrations of synthetic trypsin inhibitor bisbenzamidine affects negatively the insect performance, reduce the alimentary consumption, the digestibility, the assimilation efficiency of the food ingested and digested, the insect proteolytic activity and protein digestibility. To test these hypotheses were realized bioassays with individualized caterpillars and supplied daily with diets containing 0; 0,00095; 0,0019; 0,0038; 0,0076; 0,0152; 0,0304; 0,0608; 0,0912; 0,125 e 0,25% (w/w) of berenil during the entire larval phase. The digestion indices, the duration of larval phase and life cycle, the accumulated survival and the average of time survival, the pupae and adults weight, the body and wings of the adults moths were determined. Additionally, were evaluated the protein digestibility and the enzymatic activity of midgut extracts of A. gemmatalis exposed to berenil in diet, in the same concentrations above. All parameters assessed of life history were negatively affected with the increase of inhibitors in diet. Were verified an increase in the larval phase and life cycle duration, reduction in the pupae weight and body and wings length. There was reduction in the incorporated biomass and the relative growth rate. Otherwise, there were increases in ingestion, approximated digestion, and relative diet consumption rate and in the ingestion and excretion balance. The diet individual consumption increased until the 0.0076% of berenil, diminished with the increase of inhibitor concentration. The protein digestibility was directly proportional to berenil concentration while the proteolytic, amidolytic and esterolytic activities diminished with the increasing of inhibitor concentration. Therefore, albeit the berenil mixed in increasing concentrations in diet affects negatively in all life history parameters of insect, the caterpillars improve the diet intake. However, even so the total digestibility and the protein digestibility increase in higher inhibitor concentrations, the insect show lower enzymatic activity and, consequently, reduction in the efficiency in assimilation of ingested and digested food, indicating that the insect did not show adaptative mechanisms to prevent the inhibition of tripsina-like enzymes existents in your digestive tract. Thus, the berenil is a promising strategy to new studies with the subject of to synthesis mimicry peptides to apply in the field to control A. gemmatalis caterpillars in the soybean crops.Inibidores de proteases podem reduzir a eficiência digestiva, retardar o desenvolvimento e diminuir a atividade proteolítica do inseto, resultando em aumento da mortalidade pela deficiência de aminoácidos livres para a manutenção das funções vitais. Este trabalho teve por objetivos avaliar se concentrações crescentes do inibidor sintético de serino- proteases, berenil, presente na dieta de A. gemmatalis afeta negativamente a performance do inseto, reduz o consumo alimentar, a digestibilidade e a eficiência na assimilação do alimento ingerido e digerido, reduz a atividade proteolítica e tríptica do inseto e reduz sua digestibilidade protéica. Para testar essa hipótese foram realizados bioensaios com lagartas individualizadas e alimentadas diariamente em dietas contendo 0; 0,00095; 0,0019; 0,0038; 0,0076; 0,0152; 0,0304; 0,0608; 0,0912; 0,125 e 0,25% (p/p) de berenil durante toda a fase larval. Foram determinados os índices digestórios, a duração do ciclo de vida e da fase larval, a sobrevivência acumulada e o tempo médio de vida, o peso das pupas e dos adultos, o tamanho corporal e das asas das mariposas adultas. Adicionalmente, foram avaliadas a digestibilidade protéica e a atividade enzimática de extratos do intestino médio de A. gemmatalis submetidas ao berenil na dieta, nas concentrações determinadas acima. Todos os parâmetros avaliados da história de vida do inseto foram afetados negativamente com o aumento da concentração do inibidor na dieta. Foi verificado aumento na duração da fase larval e do ciclo de vida, diminuição no peso das pupas, adultos, tamanho dos adultos e comprimento das asas. Houve redução na biomassa incorporada e na taxa relativa de crescimento. Por outro lado, houve aumento na ingestão, digestibilidade aproximada, na taxa de consumo relativa da dieta e no balanço entre ingestão e excreção das fezes. O consumo individual total da dieta aumentou até a concentração de 0,0076% do inibidor na dieta, decrescendo com o aumento da concentração de inibidor. A digestibilidade protéica foi diretamente proporcional à concentração de berenil enquanto as atividades proteolítica, amidásica e esterásica diminuíram com o aumento da concentração do inibidor. Desse modo, embora a presença de berenil em concentrações crescentes na dieta interfira negativamente em todos os parâmetros da história de vida do inseto, as lagartas respondem aumentando a ingestão da dieta. Contudo, mesmo que a digestibilidade total e a digestibilidade protéica aumentem em concentrações maiores de inibidor, o inseto apresenta menor atividade enzimática e, conseqüentemente, baixa eficiência na assimilação do alimento ingerido e digerido, indicando que o inseto não demonstra mecanismos adaptativos para impedir a inibição das enzimas tripsina-like presentes em seu sistema digestivo. Nesse sentido, o berenil surge como estratégia promissora para novos estudos visando a síntese de peptídeos miméticos para aplicação no campo como forma de controle de A. gemmatalis nos cultivos de soja.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em EntomologiaUFVBRCiência entomológica; Tecnologia entomológicaAnticarsia gemmatalisSojaEnzimas digestivasAnticarsia gemmatalisSoybeanDigestive enzymesCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIAEfeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae)Effect of serine-proteases inhibitor, berenil, on the feeding efficiency, proteolytic activity and post- embryonic development of Anticarsia gemmatalis (Lepidoptera: Noctuidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1012402https://locus.ufv.br//bitstream/123456789/3870/1/texto%20completo.pdfe93e297eb37b3a24f8504893d77af531MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain197654https://locus.ufv.br//bitstream/123456789/3870/2/texto%20completo.pdf.txt6e6edce6e5d42d362617db402f2834cbMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3827https://locus.ufv.br//bitstream/123456789/3870/3/texto%20completo.pdf.jpg4be98a6e5b79160f30d4ff05f9f07a86MD53123456789/38702016-04-09 23:22:14.408oai:locus.ufv.br:123456789/3870Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-10T02:22:14LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
dc.title.alternative.eng.fl_str_mv |
Effect of serine-proteases inhibitor, berenil, on the feeding efficiency, proteolytic activity and post- embryonic development of Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
spellingShingle |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) Moreira, Lílian Fernandes Anticarsia gemmatalis Soja Enzimas digestivas Anticarsia gemmatalis Soybean Digestive enzymes CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA |
title_short |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_full |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_fullStr |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
title_sort |
Efeito do inibidor de serino-proteases, berenil, sobre a eficiência alimentar, atividade proteolítica e desenvolvimento pós-embrionário de Anticarsia gemmatalis (Lepidoptera: Noctuidae) |
author |
Moreira, Lílian Fernandes |
author_facet |
Moreira, Lílian Fernandes |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7479025604827544 |
dc.contributor.author.fl_str_mv |
Moreira, Lílian Fernandes |
dc.contributor.advisor-co1.fl_str_mv |
Campos, Wellington Garcia |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793941J6 |
dc.contributor.advisor-co2.fl_str_mv |
Oliveira, Joel Antônio de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707224A0 |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Maria Goreti de Almeida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790894D6 |
dc.contributor.referee1.fl_str_mv |
Andrade, Milton Hércules Guerra de |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788176U9 |
dc.contributor.referee2.fl_str_mv |
Guia, Thiago Rennó dos Mares |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763600P2 |
contributor_str_mv |
Campos, Wellington Garcia Oliveira, Joel Antônio de Oliveira, Maria Goreti de Almeida Andrade, Milton Hércules Guerra de Guia, Thiago Rennó dos Mares |
dc.subject.por.fl_str_mv |
Anticarsia gemmatalis Soja Enzimas digestivas |
topic |
Anticarsia gemmatalis Soja Enzimas digestivas Anticarsia gemmatalis Soybean Digestive enzymes CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA |
dc.subject.eng.fl_str_mv |
Anticarsia gemmatalis Soybean Digestive enzymes |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA |
description |
Protease inhibitors can reduce the digestive efficiency, to delay the development and to diminish the insect proteolytic activity resulting in increase of mortality through deficiency of free amino acids for maintenance of vital functions. The subject of this work were to evaluate if A. gemmatalis larvae feeding in artificial diets containing increasing concentrations of synthetic trypsin inhibitor bisbenzamidine affects negatively the insect performance, reduce the alimentary consumption, the digestibility, the assimilation efficiency of the food ingested and digested, the insect proteolytic activity and protein digestibility. To test these hypotheses were realized bioassays with individualized caterpillars and supplied daily with diets containing 0; 0,00095; 0,0019; 0,0038; 0,0076; 0,0152; 0,0304; 0,0608; 0,0912; 0,125 e 0,25% (w/w) of berenil during the entire larval phase. The digestion indices, the duration of larval phase and life cycle, the accumulated survival and the average of time survival, the pupae and adults weight, the body and wings of the adults moths were determined. Additionally, were evaluated the protein digestibility and the enzymatic activity of midgut extracts of A. gemmatalis exposed to berenil in diet, in the same concentrations above. All parameters assessed of life history were negatively affected with the increase of inhibitors in diet. Were verified an increase in the larval phase and life cycle duration, reduction in the pupae weight and body and wings length. There was reduction in the incorporated biomass and the relative growth rate. Otherwise, there were increases in ingestion, approximated digestion, and relative diet consumption rate and in the ingestion and excretion balance. The diet individual consumption increased until the 0.0076% of berenil, diminished with the increase of inhibitor concentration. The protein digestibility was directly proportional to berenil concentration while the proteolytic, amidolytic and esterolytic activities diminished with the increasing of inhibitor concentration. Therefore, albeit the berenil mixed in increasing concentrations in diet affects negatively in all life history parameters of insect, the caterpillars improve the diet intake. However, even so the total digestibility and the protein digestibility increase in higher inhibitor concentrations, the insect show lower enzymatic activity and, consequently, reduction in the efficiency in assimilation of ingested and digested food, indicating that the insect did not show adaptative mechanisms to prevent the inhibition of tripsina-like enzymes existents in your digestive tract. Thus, the berenil is a promising strategy to new studies with the subject of to synthesis mimicry peptides to apply in the field to control A. gemmatalis caterpillars in the soybean crops. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-07-28 |
dc.date.available.fl_str_mv |
2009-02-16 2015-03-26T13:30:20Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:30:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MOREIRA, Lílian Fernandes. Effect of serine-proteases inhibitor, berenil, on the feeding efficiency, proteolytic activity and post- embryonic development of Anticarsia gemmatalis (Lepidoptera: Noctuidae). 2007. 123 f. Dissertação (Mestrado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2007. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/3870 |
identifier_str_mv |
MOREIRA, Lílian Fernandes. Effect of serine-proteases inhibitor, berenil, on the feeding efficiency, proteolytic activity and post- embryonic development of Anticarsia gemmatalis (Lepidoptera: Noctuidae). 2007. 123 f. Dissertação (Mestrado em Ciência entomológica; Tecnologia entomológica) - Universidade Federal de Viçosa, Viçosa, 2007. |
url |
http://locus.ufv.br/handle/123456789/3870 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Entomologia |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ciência entomológica; Tecnologia entomológica |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/3870/1/texto%20completo.pdf https://locus.ufv.br//bitstream/123456789/3870/2/texto%20completo.pdf.txt https://locus.ufv.br//bitstream/123456789/3870/3/texto%20completo.pdf.jpg |
bitstream.checksum.fl_str_mv |
e93e297eb37b3a24f8504893d77af531 6e6edce6e5d42d362617db402f2834cb 4be98a6e5b79160f30d4ff05f9f07a86 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1794528623211839488 |