Antígenos de larvas de Taenia crassiceps e Taenia solium em teste ELISA para diagnóstico da cisticercose bovina

• Ano de defesa: 2004
• Autor(a) principal: Monteiro, Lílian Lameck
• Orientador(a): Pinto, Paulo Sérgio de Arruda
• Banca de defesa: Salcedo, Joaquín Hernán Patarroyo , Bevilacqua, Paula Dias , Araújo, Jackson Victor de , Santos, Wagner Luiz Moreira dos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Cisticercose; Bovino; Parasito; Taenia crassiceps; Taenia solium; Taenia saginata; Teste imunoenzimático
• Palavras-chave em Inglês: Cysticercosis; Bovine; Parasite; Taenia crassiceps; Taenia solium; Taenia saginata; Enzyme-linked immunosorbent assay
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::INSPECAO DE PRODUTOS DE ORIGEM ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/4990

This study was carried out with the purpose to develop an serological diagnosis protocol of bovine cysticercosis for the ELISA test using two metacestodes antigens of Taenia crassiceps and three of Taenia solium. There were used 20 sera of experimentally infected cattle with T. saginata eggs, 60 of cattle with natural infection, diagnosed at slaughterhouses, five of cattle negative for cisticercose, reared in isolation, 55 of negative bovine in slaughterhouses and 10 of bovine with actinomicosis (n=2), actinobacilosis (n=1), fasciolosis (n=1) diagnosed at slaughterhouse and of experimentally infected cattle with Anaplasma marginale (n=3), Babesia sp. (n=2) and concomitant infection for Anaplasma marginale and Babesia bovis (n=1). There were used the total and fluid cistic antigens of T. Crassiceps metacestodes and escólex and membrane antigens of T. solium metacestodes in ELISA test, after previous characterization in polyacrylamide gel electrophoresis (PAGE) in gradient 5 to 20%. The better antigen concentration was 1 µg and the better sera and conjugated dilutions were 1:25 and 1:5.000, respectively. Although the T. solium antigens have provided the most sensibility values, the T. crassiceps antigens also showed good performance to the bovine cysticercosis diagnosis. Different control serum groups employed for the cut-off calculation had changed the ELISA test results. We can concluded that the ELISA test for antibodies detection presents deficiencies in the diagnosis of naturally infected animals that showed low sensibility (5 to 32%) for different antigens. However, for experimentally infected cattle, the sensibility was high, 75 to 90% for different antigens. The test could still be considered useful in the differentiation between the cisticercose and other diseases, due to its high specificity rates (81 to 100%).