Diversidade molecular de Phakopsora pachyrhizi em genótipos de soja com diferentes genes de resistência a ferrugem

• Ano de defesa: 2013
• Autor(a) principal: Jorge, Vanderson Ricardo
• Orientador(a): Oliveira, Luiz Orlando de
• Banca de defesa: Pereira, Olinto Liparini
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Genética e Melhoramento
• Departamento: Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
• País: BR
• Palavras-chave em Português: Ferrugem asiática; Diversidade genética
• Palavras-chave em Inglês: Asian rust; Genetic diversity
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
• Link de acesso: http://locus.ufv.br/handle/123456789/4781

The Asian Soybean Rust is a major disease of soybean causing enormous economic damage to agribusiness. Rust resistance genes have been reported, but the resistance of the cultivars is still partial. The objective of the research was to assess the diversity of the fungus P. pachyrhizi from environmental samples originating from experiments cultivated with susceptible genotypes and genotypes with rust resistance gene. The gene regions of internal transcribed spacer (ITS) and the gene for Factor ADP ribosylation were amplified, cloned and sequenced. The sequences obtained were assembled into databases for both regions under study. The results of the analysis revealed the presence of six haplotypes for the region of ADP and 14 ribotypes for the ITS region, being similarly distributed among soybean genotypes with different tolerance levels. Population structure analysis demonstrate that genetic variability for P. pachyrhizi focuses on populations within the same location, with no significant differences between evaluated locations. The presence of genes which grant resistance to some genotypes did not affect the distribution of ribotypes to the locations under consideration. The similarity between different location samples and different genotypes indicate an intense gene flow than can be result of a long distance spore dispersion by the wind.