Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Silva, Jeferson Júnior Da lattes
Orientador(a): Chavasco, Jorge Kleber lattes
Banca de defesa: Fiorini, João Evangelista, Cabral, Maulori Curié
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Alfenas
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
Departamento: Instituto de Ciências Biomédicas
País: Brasil
Palavras-chave em Português:
Extratos Vegetais
Produtos com ação antimicrobiana
MRSA
Testes de Toxicidade
Testes de Sensibilidade Microbiana
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
Link de acesso: https://repositorio.unifal-mg.edu.br/handle/123456789/287
Resumo: Currently S. aureus methicillin resistant (MRSA) is the most important nosocomial pathogen due to multidrug resistance to available antimicrobials, which directs efforts in the search for new therapies. In this study we evaluated the in vitro antimicrobial activity of plant extracts against ambient aerial isolates of MRSA previously identified. The hydroethanolic extracts of Annona crassiflora, Bidens pilosa, Eugenia pyriformis, Heliconia rostrata and Plinia cauliflora were obtained by steeping in ethyl alcohol, 70%, evaporated and lyophilized. The antimicrobial activity was evaluated by agar diffusion and determination of minimum inhibitory concentration (MIC) by microdilution broth. Toxicity testing in cell culture and phytochemical analysis complemented the analysis where most of the extracts showed low toxicity, presence of alkaloids, flavonoids, tannins and saponins. The extracts of leaf, stem and fruit rind of Plinia cauliflora, stem and leaf of Eugenia pyriformis, stem, pulp, fruit rind and leaf of Annona crassiflora and flower, stem and leaf of Bidens pilosa were effective against multidrug-resistant samples analyzed being that the leaf extract of Bidens pilosa showed the best in vitro anti-MRSA activity by both methods evaluated.
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spelling Silva, Jeferson Júnior Dahttp://lattes.cnpq.br/2716662286814085Boriollo, Marcelo Fabiano GomesFiorini, João EvangelistaCabral, Maulori CuriéChavasco, Jorge Kleberhttp://lattes.cnpq.br/21256337009598222015-05-26T19:28:15Z2013-03-08SILVA, Jeferson Júnior da. Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA). 2013. 54 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Alfenas, Alfenas, MG, 2013.https://repositorio.unifal-mg.edu.br/handle/123456789/287Currently S. aureus methicillin resistant (MRSA) is the most important nosocomial pathogen due to multidrug resistance to available antimicrobials, which directs efforts in the search for new therapies. In this study we evaluated the in vitro antimicrobial activity of plant extracts against ambient aerial isolates of MRSA previously identified. The hydroethanolic extracts of Annona crassiflora, Bidens pilosa, Eugenia pyriformis, Heliconia rostrata and Plinia cauliflora were obtained by steeping in ethyl alcohol, 70%, evaporated and lyophilized. The antimicrobial activity was evaluated by agar diffusion and determination of minimum inhibitory concentration (MIC) by microdilution broth. Toxicity testing in cell culture and phytochemical analysis complemented the analysis where most of the extracts showed low toxicity, presence of alkaloids, flavonoids, tannins and saponins. The extracts of leaf, stem and fruit rind of Plinia cauliflora, stem and leaf of Eugenia pyriformis, stem, pulp, fruit rind and leaf of Annona crassiflora and flower, stem and leaf of Bidens pilosa were effective against multidrug-resistant samples analyzed being that the leaf extract of Bidens pilosa showed the best in vitro anti-MRSA activity by both methods evaluated.Atualmente, S. aureus meticilina resistente (MRSA) é o mais importante patógeno nosocomial, devido à sua multirresistência aos antimicrobianos disponíveis, o que direciona esforços na busca de novas alternativas terapêuticas. Neste estudo foi avaliada a ação antimicrobiana de extratos vegetais contra isolados MRSA do ambiente aéreo, identificados previamente. Os extratos de Annona crassiflora, Bidens pilosa, Eugenia pyriformis, Heliconia rostrata e Plinia cauliflora foram obtidos por maceração em álcool etílico a 70%, submetidos à rota evaporador e liofilizados. Estes foram testados quanto à ação antimicrobiana pela técnica de difusão em ágar. Posteriormente, realizou-se a concentração inibitória mínima (CIM) pela técnica de microdiluição em caldo. Testes de toxicidade em cultura celular e análise fitoquímica dos extratos complementaram a análise, onde a maioria dos extratos apresentou baixa toxicidade, presença de alcalóides, flavonóides, taninos e saponinas. Os extratos da folha, casca do fruto e caule de Plinia cauliflora, caule e folha de Eugenia pyriformis, caule, polpa, casca do fruto e folha de Annona crassiflora e flor, caule e folha de Bidens pilosa foram efetivos contra as amostras multirresistentes analisadas, sendo que o extrato de Bidens pilosa folha apresentou a melhor atividade antibacteriana in vitro por ambos os métodos avaliados.Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIGapplication/pdfporUniversidade Federal de AlfenasPrograma de Pós-Graduação em Ciências FarmacêuticasUNIFAL-MGBrasilInstituto de Ciências Biomédicasinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Extratos VegetaisProdutos com ação antimicrobianaMRSATestes de ToxicidadeTestes de Sensibilidade MicrobianaCIENCIAS BIOLOGICAS::MICROBIOLOGIATriagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion1196850848737529011600600600-3854583469976220812-1527361517405938873reponame:Repositório Institucional da Universidade Federal de Alfenas - RiUnifalinstname:Universidade Federal de Alfenas (UNIFAL)instacron:UNIFALSilva, Jeferson Júnior DaLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt-BR.fl_str_mv Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
title Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
spellingShingle Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
Silva, Jeferson Júnior Da
Extratos Vegetais
Produtos com ação antimicrobiana
MRSA
Testes de Toxicidade
Testes de Sensibilidade Microbiana
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
title_short Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
title_full Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
title_fullStr Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
title_full_unstemmed Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
title_sort Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA)
author Silva, Jeferson Júnior Da
author_facet Silva, Jeferson Júnior Da
author_role author
dc.contributor.author.fl_str_mv Silva, Jeferson Júnior Da
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2716662286814085
dc.contributor.advisor-co1.fl_str_mv Boriollo, Marcelo Fabiano Gomes
dc.contributor.referee1.fl_str_mv Fiorini, João Evangelista
dc.contributor.referee2.fl_str_mv Cabral, Maulori Curié
dc.contributor.advisor1.fl_str_mv Chavasco, Jorge Kleber
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2125633700959822
contributor_str_mv Boriollo, Marcelo Fabiano Gomes
Fiorini, João Evangelista
Cabral, Maulori Curié
Chavasco, Jorge Kleber
dc.subject.por.fl_str_mv Extratos Vegetais
Produtos com ação antimicrobiana
MRSA
Testes de Toxicidade
Testes de Sensibilidade Microbiana
topic Extratos Vegetais
Produtos com ação antimicrobiana
MRSA
Testes de Toxicidade
Testes de Sensibilidade Microbiana
CIENCIAS BIOLOGICAS::MICROBIOLOGIA
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::MICROBIOLOGIA
description Currently S. aureus methicillin resistant (MRSA) is the most important nosocomial pathogen due to multidrug resistance to available antimicrobials, which directs efforts in the search for new therapies. In this study we evaluated the in vitro antimicrobial activity of plant extracts against ambient aerial isolates of MRSA previously identified. The hydroethanolic extracts of Annona crassiflora, Bidens pilosa, Eugenia pyriformis, Heliconia rostrata and Plinia cauliflora were obtained by steeping in ethyl alcohol, 70%, evaporated and lyophilized. The antimicrobial activity was evaluated by agar diffusion and determination of minimum inhibitory concentration (MIC) by microdilution broth. Toxicity testing in cell culture and phytochemical analysis complemented the analysis where most of the extracts showed low toxicity, presence of alkaloids, flavonoids, tannins and saponins. The extracts of leaf, stem and fruit rind of Plinia cauliflora, stem and leaf of Eugenia pyriformis, stem, pulp, fruit rind and leaf of Annona crassiflora and flower, stem and leaf of Bidens pilosa were effective against multidrug-resistant samples analyzed being that the leaf extract of Bidens pilosa showed the best in vitro anti-MRSA activity by both methods evaluated.
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-08
dc.date.accessioned.fl_str_mv 2015-05-26T19:28:15Z
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dc.identifier.citation.fl_str_mv SILVA, Jeferson Júnior da. Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA). 2013. 54 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Alfenas, Alfenas, MG, 2013.
dc.identifier.uri.fl_str_mv https://repositorio.unifal-mg.edu.br/handle/123456789/287
identifier_str_mv SILVA, Jeferson Júnior da. Triagem antimicrobiana de extratos vegetais frente a isolados ambientais de Staphylococcus aureus Oxacilina resistentes (ORSA). 2013. 54 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Alfenas, Alfenas, MG, 2013.
url https://repositorio.unifal-mg.edu.br/handle/123456789/287
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dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Ciências Biomédicas
publisher.none.fl_str_mv Universidade Federal de Alfenas
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