Caracterização química e estudos pré-clínicos de segurança e eficácia de brácteas da Bougainvillea glabra Choisy

• Ano de defesa: 2024
• Autor(a) principal: Caurio, Aline Castro
• Orientador(a): Denardin, Elton Luis Gasparotto
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Pampa
• Programa de Pós-Graduação: Doutorado em Bioquímica
• Departamento: Campus Uruguaiana
• País: Brasil
• Palavras-chave em Português: Drosophila melanogaster; ratos, produtos naturais; Bougainvillea glabra; Natural products; C6 glial cells; Células gliais C6; Rats
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.unipampa.edu.br/handle/123456789/11292

Natural products have historically been used to treat different diseases as a complementary or protagonist therapy. It is already proven that natural products have several bioactive compounds with antioxidant capacity that justify the effectiveness of their use. Bougainvillea glabra Choisy is a plant whose antioxidant activity has already been reported in the literature. The present work aims to investigate the pharmacological and toxicological potential of the hydroalcoholic extract of bracts of Bougainvillea glabra Choisy (EBBGC), an ornamental plant, native to Brazil, by means of three studies: in chapter I we evaluate the protective effect of the extract against Paraquat-induced toxicity (PQ) in Drosophila melanogaster; in chapter II (first manuscript) we evaluate the acute effect of the bract extract of Bougainvillea glabra Choisy (EBBGC), an ornamental plant native to Brazil. Bougainvillea glabra in Wistar rats following the OECD 423 guideline; and in chapter III (second manuscript) we evaluated the effect and possible antineuroinflammatory mechanisms of EBBGC against co-exposure to LPS in C6 glial cell culture. The results obtained and presented in this thesis demonstrate that the bract extract of Bougainvillea glabra has several bioactive compounds that act by capturing free radicals and reducing the oxidative picture. In Drosophila melanogaster, EBBGC35 had a protective effect against PQ on the head and body of flies, attenuated the behavior by blocking oxidative damage, exerted antioxidant effects and decreased the production of reactive species. Thus, low-dose BGC suggests an alternative for the treatment of diseases related to oxidative stress. In rodents, the EBBGC55 exerted antioxidant effects, being classified as a non-toxic substance through the classification of the Glabally Harmonized System (GHS) as belonging to category 5 (GHS = 5). In addition, the extract caused a decrease in triglyceride levels, an increase in HDL levels and an increase in hepatic and renal oxidative damage in some concentrations tested, but the antioxidant enzymes were positively modulated, causing integrity in the architecture and functioning of the tissues. In C6 glial cells EBBGC55 was able to reverse oxidative damage of lipids and proteins, modulate the activity of antioxidant enzymes, showing a promising effect on the reduction of pro-inflammatory cytokines as well as LPS-activated neuroinflammatory pathways in C6 glial cells. With this, EBBGC55 may be able to effectively alleviate inflammatory diseases by being an inhibitor of MAPK p38 and being considered a potent new drug candidate for inflammation-related diseases.