Ocorrência de Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) em Achatina fulica (Mollusca, Gastropoda) na Baixada Santista

• Ano de defesa: 2014
• Autor(a) principal: Guerino, Laura Rocha [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Meningoencefalite; Sistema nervoso central - Doenças; Molusco; Larva; Reação em cadeia de polimerase; Meningoencephalitis
• Link de acesso: http://hdl.handle.net/11449/124068; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/11-05-2015/000828361.pdf

Angiostrongylus cantonensis is the causative agent of eosinophilic meningoencephalitis, also called rat lungworm. Worldwide, the expansion of this nematode is linked to the dispersal and colonization by giant African snail (Achatina fulica). The aim of this work was to verify the occurrence of larvae of A. cantonensis in mollusks A. fulica naturally infected in nine cities that compose the Baixada Santista region. About 540 specimens were collected in urban areas of the following cities: São Vicente, Santos, Praia Grande, Cubatão, Mongaguá, Itanhaém, Peruibe, Guarujá and Bertioga. The technique used for obtaining larvae was based on Wallace & Rosen and sedimentation for 3 h using the method of Baermann. After sedimentation, the material was observed under bright-field stereoscopic microscope for viewing larvae that were subsequently frozen at -70°C. DNA was extracted and submitted to PCR - RFLP (polymerase chain reaction and restriction fragment length polymorphism) directed to the internal transcribed spacer region two (ITS2 - rDNA) and cleaved with ClaI enzyme. For morphological and morphometric analyses, 60 larvae were used, 30 in stage L2 and 30 in stage L3. Overall, 90 points (neighborhoods) were analyzed, of which 73 (81.11 %) showed mollusks with nematode larvae. Of the 540 snails examined, 204 (37.77 %) showed nematode larvae, and 79 (38.72 %) were submitted to molecular analyses, and infection by A. cantonensis was found in 63 (79.75 %) mollusks. The number of larvae per mollusk varied from 1 to 9723 in specimens showing 61.0 and 70.0 mm in shell length, respectively. No significant differences were observed between number of larvae of A. cantonensis and the shell length of young adults and adults, nor between the average number of larvae found between young adults and adults. Significant differences were found when comparing the number of young adult and adult individuals ...