Caracterização química e potencial biotecnológico do extrato de alcachofra (Cynara scolymus L.) no tratamento de sementes

• Ano de defesa: 2020
• Autor(a) principal: Bernardi, Caliandra
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Tecnológica Federal do Paraná; Ponta Grossa; Brasil; Programa de Pós-Graduação em Biotecnologia; UTFPR
• Palavras-chave em Português: Alcachofra; Fungicidas; Bactericidas; Antioxidantes; Sementes - Doenças e pragas; Artichokes; Fungicides; Bactericides; Antioxidants; Seed pathology; CNPQ::CIENCIAS BIOLOGICAS; Biotecnologia
• Link de acesso: http://repositorio.utfpr.edu.br/jspui/handle/1/4949

Research on new forms of antimicrobial agents has been gaining more importance in recent years, with emphasis on the use of plant species as a means of control, especially medicinal plants, as they mostly have compounds with antimicrobial capacity. Therefore, and seeking an alternative control of fungi and bacteria, the objective of this work was to evaluate the chemical, antioxidant, antimicrobial, biological, biochemical and physiological potential of artichoke extract (Cynara scolymus L.) in seed treatment. The research was carried out in research laboratories at UTFPR campuses in Dois Vizinhos and Pato Branco. The artichoke extract was obtained with different times and solvents. The antioxidant potential was assessed using the methodology of DPPH, ABTS and Total Phenolic Compounds. The chemical potential of the artichoke extract was evaluated through chromatographic analysis by CG / MS. To evaluate the antimicrobial activity of the extracts, the Minimum Inhibitory Concentration (MIC) and spot-test methods were used. As biological potential, the toxicity of the extract was assessed in nausea of Artemia salina and Eruca sativa seedlings and from the concentration found in the MIC technique. To evaluate the physiological potential, the extract was tested as a treatment for corn seeds (Zea mays) and soybeans (Glycine max). The results showed that the artichoke extract has antioxidant, antimicrobial potential, does not have high phytotoxicity and can be used in the treatment of storage seeds against phytopathogens.