Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia
Ano de defesa: | 2018 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Odontologia
|
Departamento: |
Escola de Ci?ncias da Sa?de
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede2.pucrs.br/tede2/handle/tede/7894 |
Resumo: | Objective: The present study aimed to evaluate the effects of systemic ozonotherapy on bone remodeling of critical defects in the calotte of rats, with and without a xenograft presence, using an animal model of immunosuppression induced by corticosteroids. Methods: Sixty male Wistar rats (180-220 g), distributed in 8 experimental groups (N = 8 / group) were used. For corticosteroid therapy (groups II, IV, VI and VIII), the animals received dexamethasone (1 mg / kg; i.p.), one injection every 48 h, starting two weeks before the surgical procedures, extending to euthanasia. Control animals (groups I, III, V and VII) received saline solution (10 ml / kg, i.p.) at the same time intervals. After two weeks of corticosteroid therapy, the animals were anesthetized with ketamine and xylazine (100 and 10 mg / kg, i.p.) to produce two critical defects in the parietal bones of the skull cap (5 mm diameter). The defects were filled with clot (groups I, II, V and VI) or Bio-Oss x xenograft (Geistlish Biomaterials, Germany) (groups III, IV, VII and VIII). In all groups, the right defect was covered by a Bio-Gide? collagen membrane (Geistlish Biomaterials, Germany). For systemic ozono-therapy, animals of groups V, VI, VII and VIII received an application of ozone (0.7 mg / kg, i.p.) every day for 7 days, starting immediately after surgery. Four weeks after the defects were created, the animals were euthanized, and the skull caps were collected for histological evaluation of the bone neoformation with hematoxylin and eosin (HE) staining. The protocols were approved by the Ethics Committee on the Use of Animals (7691). Qualitative histological analyzes were performed based on the pattern of connective tissue formation around the defect, existence of inflammatory cells in the region, aspect of bone trabeculated, osteoblastic activity around the bone matrix and existence of graft particles in the region. Results: Groups V and VII presented greater bone areas along the surface of the defect compared to VI and VIII, because of the potential effects of ozone on bone remodeling, minimizing negative interference in the glucocorticoid bone microarray. The presence of the xenografts groups III, IV, VII, VIII, provided the covering of an extensive area of the defect, forming a more prominent immature bone matrix near the edges of the defects, with the presence of graft particles dispersed in the central area. The presence of the type I collagen membrane was shown to be an important tool in the acceleration of bone remodeling used in all experimental groups, in which, in comparison to contralateral defects in which the membrane was not used, a significant increase of bone just graduated. At the systemic level, the results of ozone treatment allowed the reduction of adverse effects of glucocorticoids, such as the reduction of the body weight of the animals and atrophy of lymphatic organs such as spleen, observed in the groups treated alone with dexamethasone. Conclusion: Given the limitations of the animal study, the use of systemic ozone associated with the xenograft stimulates bone remodeling in critical defects of immunosuppressed rats. |
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Campos, Maria Marthahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798056Z6http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4498641Z4Louzada, Guilherme Pivatto2018-03-21T12:03:36Z2018-01-12http://tede2.pucrs.br/tede2/handle/tede/7894Objective: The present study aimed to evaluate the effects of systemic ozonotherapy on bone remodeling of critical defects in the calotte of rats, with and without a xenograft presence, using an animal model of immunosuppression induced by corticosteroids. Methods: Sixty male Wistar rats (180-220 g), distributed in 8 experimental groups (N = 8 / group) were used. For corticosteroid therapy (groups II, IV, VI and VIII), the animals received dexamethasone (1 mg / kg; i.p.), one injection every 48 h, starting two weeks before the surgical procedures, extending to euthanasia. Control animals (groups I, III, V and VII) received saline solution (10 ml / kg, i.p.) at the same time intervals. After two weeks of corticosteroid therapy, the animals were anesthetized with ketamine and xylazine (100 and 10 mg / kg, i.p.) to produce two critical defects in the parietal bones of the skull cap (5 mm diameter). The defects were filled with clot (groups I, II, V and VI) or Bio-Oss x xenograft (Geistlish Biomaterials, Germany) (groups III, IV, VII and VIII). In all groups, the right defect was covered by a Bio-Gide? collagen membrane (Geistlish Biomaterials, Germany). For systemic ozono-therapy, animals of groups V, VI, VII and VIII received an application of ozone (0.7 mg / kg, i.p.) every day for 7 days, starting immediately after surgery. Four weeks after the defects were created, the animals were euthanized, and the skull caps were collected for histological evaluation of the bone neoformation with hematoxylin and eosin (HE) staining. The protocols were approved by the Ethics Committee on the Use of Animals (7691). Qualitative histological analyzes were performed based on the pattern of connective tissue formation around the defect, existence of inflammatory cells in the region, aspect of bone trabeculated, osteoblastic activity around the bone matrix and existence of graft particles in the region. Results: Groups V and VII presented greater bone areas along the surface of the defect compared to VI and VIII, because of the potential effects of ozone on bone remodeling, minimizing negative interference in the glucocorticoid bone microarray. The presence of the xenografts groups III, IV, VII, VIII, provided the covering of an extensive area of the defect, forming a more prominent immature bone matrix near the edges of the defects, with the presence of graft particles dispersed in the central area. The presence of the type I collagen membrane was shown to be an important tool in the acceleration of bone remodeling used in all experimental groups, in which, in comparison to contralateral defects in which the membrane was not used, a significant increase of bone just graduated. At the systemic level, the results of ozone treatment allowed the reduction of adverse effects of glucocorticoids, such as the reduction of the body weight of the animals and atrophy of lymphatic organs such as spleen, observed in the groups treated alone with dexamethasone. Conclusion: Given the limitations of the animal study, the use of systemic ozone associated with the xenograft stimulates bone remodeling in critical defects of immunosuppressed rats.Objetivo: O presente estudo teve por objetivo avaliar os efeitos da ozonioterapia sist?mica sobre o remodelamento ?sseo de defeitos cr?ticos na calota de ratos, com e sem a presen?a de xenoenxerto, utilizando um modelo animal de imunossupress?o induzido por corticoterapia. M?todos: Foram utilizados 64 ratos machos Wistar (180-220 g), distribu?dos em 8 grupos experimentais (N = 8/grupo). Para a corticoterapia (grupos II, IV, VI e VIII), os animais receberam dexametasona (1 mg/kg; i.p.), uma inje??o a cada 48 h, com in?cio duas semanas antes dos procedimentos cir?rgicos, se estendendo at? a eutan?sia. Os animais controles (grupos I, III, V e VII) receberam solu??o salina (10 ml/kg; i.p.), nos mesmos intervalos de tempo. Ap?s duas semanas do in?cio da corticoterapia, os animais foram anestesiados com quetamina e xilazina (100 e 10 mg/kg; i.p.), para a confec??o de dois defeitos cr?ticos nos ossos parietais da calota craniana (5 mm de di?metro). Os defeitos foram preenchidos com co?gulo (grupos I, II, V e VI) ou com xenoenxerto Bio-Oss? (Geistlish Biomaterials, Germany) (grupos III, IV, VII e VIII). Em todos os grupos, o defeito da direita foi coberto por uma membrana de col?geno Bio-Gide? (Geistlish Biomaterials, Germany). Para a ozonioterapia sist?mica, os animais dos grupos V, VI, VII e VIII receberam uma aplica??o de oz?nio (0,7 mg/kg; i.p.), todos os dias, durante 7 dias, iniciando imediatamente ap?s a cirurgia. Decorridos quatro semanas da cria??o dos defeitos, os animais foram eutanasiados e as calotas cranianas foram coletadas para avalia??o histol?gica da neoforma??o ?ssea, com colora??o de hematoxilina e eosina (HE). Os protocolos foram aprovados pela Comiss?o de ?tica no Uso de Animais (7691). Foram realizadas an?lises histol?gicas qualitativas baseado no aspecto do trabeculado ?sseo, atividade osteobl?stica em torno da matriz ?ssea, padr?o de forma??o de tecido conjuntivo na ?rea do defeito, e exist?ncia de part?culas de enxerto na regi?o. Resultados: Os grupos V, VII apresentaram maiores ?reas ?sseas ao longo da superf?cie do defeito, em compara??o com os VI e VIII, como consequ?ncia dos efeitos potenciais do oz?nio no remodelamento ?sseo, minimizando as interfer?ncias negativas na microarquitetura ?ssea causada pelos glicocortic?ides. A presen?a do xenoenxerto nos grupos III, IV, VII, VIII, proporcionou o recobrimento de extensa ?rea do defeito, formando matriz ?ssea imatura mais proeminente nas proximidades dos bordos dos defeitos, com presen?a de part?culas de enxerto dispersas na ?rea central. A presen?a da membrana de col?geno tipo I, se mostrou um importante recurso na acelera??o do remodelamento ?sseo utilizado em todos os grupos experimentais, na qual em compara??o com os defeitos contralaterais em que n?o foi utilizado a membrana, pode ser visto um significativo aumento de osso rec?m-formado. A n?vel sist?mico, os resultados do tratamento com oz?nio possibilitaram a diminui??o de efeitos adversos dos glicocorticoides, tais como a diminui??o do peso corporal dos animais e atrofia de ?rg?os linf?ticos como o ba?o, observados nos grupos tratados isoladamente com a dexametasona. Conclus?o: Diante das limita??es do estudo animal, a utiliza??o de oz?nio sist?mico associado ao xenoenxerto, estimula o remodelamento ?sseo em defeitos cr?ticos de ratos imunossuprimidos.Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-13T18:14:07Z No. of bitstreams: 1 GUILHERME_PIVATTO_LOUZADA_DIS.pdf: 4084164 bytes, checksum: 92327e9e0d6d6a7868235af68dc557f1 (MD5)Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-21T11:56:19Z (GMT) No. of bitstreams: 1 GUILHERME_PIVATTO_LOUZADA_DIS.pdf: 4084164 bytes, checksum: 92327e9e0d6d6a7868235af68dc557f1 (MD5)Made available in DSpace on 2018-03-21T12:03:36Z (GMT). No. of bitstreams: 1 GUILHERME_PIVATTO_LOUZADA_DIS.pdf: 4084164 bytes, checksum: 92327e9e0d6d6a7868235af68dc557f1 (MD5) Previous issue date: 2018-01-12Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/171203/DIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.jpghttps://tede2.pucrs.br/tede2/retrieve/186770/DIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em OdontologiaPUCRSBrasilEscola de Ci?ncias da Sa?deOz?nioGlicocortic?idesOsteoporoseImunossupress?oRegenera??o ?sseaCIENCIAS DA SAUDE::ODONTOLOGIAAvalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTrabalho ser? publicado como artigo ou livro60 meses21/03/2023-8096554818733665164500500600-20704984698792443492075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSORIGINALDIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdfDIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdfapplication/pdf4084164https://tede2.pucrs.br/tede2/bitstream/tede/7894/5/DIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf92327e9e0d6d6a7868235af68dc557f1MD55THUMBNAILDIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.jpgDIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.jpgimage/jpeg4091https://tede2.pucrs.br/tede2/bitstream/tede/7894/4/DIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.jpg2fc190ce9e7e7dc9484ea9a8737c08d8MD54DIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.jpgDIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.jpgimage/jpeg6106https://tede2.pucrs.br/tede2/bitstream/tede/7894/7/DIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.jpg98d603b39f4a44daeb001080f7cd2b1dMD57TEXTDIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.txtDIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.txttext/plain2357https://tede2.pucrs.br/tede2/bitstream/tede/7894/3/DIS_GUILHERME_PIVATTO_LOUZADA_CONFIDENCIAL.pdf.txt6b90f55c62da1d4c44f131c2a1f9c719MD53DIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.txtDIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.txttext/plain111989https://tede2.pucrs.br/tede2/bitstream/tede/7894/6/DIS_GUILHERME_PIVATTO_LOUZADA_COMPLETO.pdf.txt02e87a4c46bb74000bc02dc215eb4d35MD56LICENSElicense.txtlicense.txttext/plain; charset=utf-8610https://tede2.pucrs.br/tede2/bitstream/tede/7894/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/78942023-03-22 12:00:05.705oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2023-03-22T15:00:05Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
title |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
spellingShingle |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia Louzada, Guilherme Pivatto Oz?nio Glicocortic?ides Osteoporose Imunossupress?o Regenera??o ?ssea CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
title_full |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
title_fullStr |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
title_full_unstemmed |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
title_sort |
Avalia??o dos efeitos da ozonioterapia sist?mica sobre a regenera??o de defeitos ?sseos cr?ticos, com xenoenxerto bovino, em ratos submetidos ? corticoterapia |
author |
Louzada, Guilherme Pivatto |
author_facet |
Louzada, Guilherme Pivatto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Campos, Maria Martha |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798056Z6 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4498641Z4 |
dc.contributor.author.fl_str_mv |
Louzada, Guilherme Pivatto |
contributor_str_mv |
Campos, Maria Martha |
dc.subject.por.fl_str_mv |
Oz?nio Glicocortic?ides Osteoporose Imunossupress?o Regenera??o ?ssea |
topic |
Oz?nio Glicocortic?ides Osteoporose Imunossupress?o Regenera??o ?ssea CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Objective: The present study aimed to evaluate the effects of systemic ozonotherapy on bone remodeling of critical defects in the calotte of rats, with and without a xenograft presence, using an animal model of immunosuppression induced by corticosteroids. Methods: Sixty male Wistar rats (180-220 g), distributed in 8 experimental groups (N = 8 / group) were used. For corticosteroid therapy (groups II, IV, VI and VIII), the animals received dexamethasone (1 mg / kg; i.p.), one injection every 48 h, starting two weeks before the surgical procedures, extending to euthanasia. Control animals (groups I, III, V and VII) received saline solution (10 ml / kg, i.p.) at the same time intervals. After two weeks of corticosteroid therapy, the animals were anesthetized with ketamine and xylazine (100 and 10 mg / kg, i.p.) to produce two critical defects in the parietal bones of the skull cap (5 mm diameter). The defects were filled with clot (groups I, II, V and VI) or Bio-Oss x xenograft (Geistlish Biomaterials, Germany) (groups III, IV, VII and VIII). In all groups, the right defect was covered by a Bio-Gide? collagen membrane (Geistlish Biomaterials, Germany). For systemic ozono-therapy, animals of groups V, VI, VII and VIII received an application of ozone (0.7 mg / kg, i.p.) every day for 7 days, starting immediately after surgery. Four weeks after the defects were created, the animals were euthanized, and the skull caps were collected for histological evaluation of the bone neoformation with hematoxylin and eosin (HE) staining. The protocols were approved by the Ethics Committee on the Use of Animals (7691). Qualitative histological analyzes were performed based on the pattern of connective tissue formation around the defect, existence of inflammatory cells in the region, aspect of bone trabeculated, osteoblastic activity around the bone matrix and existence of graft particles in the region. Results: Groups V and VII presented greater bone areas along the surface of the defect compared to VI and VIII, because of the potential effects of ozone on bone remodeling, minimizing negative interference in the glucocorticoid bone microarray. The presence of the xenografts groups III, IV, VII, VIII, provided the covering of an extensive area of the defect, forming a more prominent immature bone matrix near the edges of the defects, with the presence of graft particles dispersed in the central area. The presence of the type I collagen membrane was shown to be an important tool in the acceleration of bone remodeling used in all experimental groups, in which, in comparison to contralateral defects in which the membrane was not used, a significant increase of bone just graduated. At the systemic level, the results of ozone treatment allowed the reduction of adverse effects of glucocorticoids, such as the reduction of the body weight of the animals and atrophy of lymphatic organs such as spleen, observed in the groups treated alone with dexamethasone. Conclusion: Given the limitations of the animal study, the use of systemic ozone associated with the xenograft stimulates bone remodeling in critical defects of immunosuppressed rats. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-03-21T12:03:36Z |
dc.date.issued.fl_str_mv |
2018-01-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://tede2.pucrs.br/tede2/handle/tede/7894 |
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http://tede2.pucrs.br/tede2/handle/tede/7894 |
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por |
language |
por |
dc.relation.program.fl_str_mv |
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dc.relation.confidence.fl_str_mv |
500 500 600 |
dc.relation.cnpq.fl_str_mv |
-2070498469879244349 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
dc.publisher.program.fl_str_mv |
Programa de P?s-Gradua??o em Odontologia |
dc.publisher.initials.fl_str_mv |
PUCRS |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Ci?ncias da Sa?de |
publisher.none.fl_str_mv |
Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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PUC_RS |
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