Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Campos, Rayane Luzia Viegas Campos lattes
Orientador(a): Parachin, N??dia Skorupa lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Cat??lica de Bras??lia
Programa de Pós-Graduação: Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia
Departamento: Escola de Sa??de e Medicina
País: Brasil
Palavras-chave em Português:
Biossurfactante
Ramnolip??deo
Produ????o
Larga escala
Saccharomyces cerevisiae
Gen??tica
Genoma
Área do conhecimento CNPq:
CIENCIAS BIOLOGICAS::GENETICA
Resumo em Inglês: Biosurfactants are metabolites that contain both hydrophobic and hydrophilic domains and are produced by various microorganisms. Due to its characteristics such molecules localize preferable in the interface of polar and non-polar solvents. Biosurfactants are widely used in pharmaceutic, cosmetic, food and agriculture industries. Moreover, they are considered advantageous when compared to chemical ones because they are biodegradable and non-toxic. Currently one of the best known biosurfactant is the rhamnolipids. However, their utilization in large scale are still not possible due to its high production costs. Rhamnolipids are produced mainly by Pseudomonas aeruginosa and are composed by one or two molecules of L-rhamnose attached to a fatty acid molecule. This study aimed the construction of yeast expression vectors that will be used to construct a recombinant strain of Saccharomyces cerevisiae efficient in the production of L-rhamnose and mono-rhamnolipid using sucrose as substrate. For the construction of these vectors, six different genes, responsible for encoding both L-rhamnose and mono-ramnolipideos biosynthetic pathways enzymes described in P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA and rhlB), and one gene, responsible for the synthesis of a substrate break enzyme, described in Pelomonas sacchraphyla (sucrose phosphorylase) were utilized. The genetic engineering proposed in this study is innovative, and has never been done before. The production of rhamnolipid in large-scale at low costs will be highly beneficial for the development of national technologies for production of high value-added chemical.
Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/1997
Resumo: Biosurfactants are metabolites that contain both hydrophobic and hydrophilic domains and are produced by various microorganisms. Due to its characteristics such molecules localize preferable in the interface of polar and non-polar solvents. Biosurfactants are widely used in pharmaceutic, cosmetic, food and agriculture industries. Moreover, they are considered advantageous when compared to chemical ones because they are biodegradable and non-toxic. Currently one of the best known biosurfactant is the rhamnolipids. However, their utilization in large scale are still not possible due to its high production costs. Rhamnolipids are produced mainly by Pseudomonas aeruginosa and are composed by one or two molecules of L-rhamnose attached to a fatty acid molecule. This study aimed the construction of yeast expression vectors that will be used to construct a recombinant strain of Saccharomyces cerevisiae efficient in the production of L-rhamnose and mono-rhamnolipid using sucrose as substrate. For the construction of these vectors, six different genes, responsible for encoding both L-rhamnose and mono-ramnolipideos biosynthetic pathways enzymes described in P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA and rhlB), and one gene, responsible for the synthesis of a substrate break enzyme, described in Pelomonas sacchraphyla (sucrose phosphorylase) were utilized. The genetic engineering proposed in this study is innovative, and has never been done before. The production of rhamnolipid in large-scale at low costs will be highly beneficial for the development of national technologies for production of high value-added chemical.
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spelling Parachin, N??dia Skorupahttp://lattes.cnpq.br/1214716137780644http://lattes.cnpq.br/3454004547074775Campos, Rayane Luzia Viegas Campos2016-12-19T18:06:48Z2015-03-31CAMPOS, Rayane Luzia Viegas Campos. Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o. 2015. 65f. Disserta????o( Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2015.https://bdtd.ucb.br:8443/jspui/handle/tede/1997Biosurfactants are metabolites that contain both hydrophobic and hydrophilic domains and are produced by various microorganisms. Due to its characteristics such molecules localize preferable in the interface of polar and non-polar solvents. Biosurfactants are widely used in pharmaceutic, cosmetic, food and agriculture industries. Moreover, they are considered advantageous when compared to chemical ones because they are biodegradable and non-toxic. Currently one of the best known biosurfactant is the rhamnolipids. However, their utilization in large scale are still not possible due to its high production costs. Rhamnolipids are produced mainly by Pseudomonas aeruginosa and are composed by one or two molecules of L-rhamnose attached to a fatty acid molecule. This study aimed the construction of yeast expression vectors that will be used to construct a recombinant strain of Saccharomyces cerevisiae efficient in the production of L-rhamnose and mono-rhamnolipid using sucrose as substrate. For the construction of these vectors, six different genes, responsible for encoding both L-rhamnose and mono-ramnolipideos biosynthetic pathways enzymes described in P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA and rhlB), and one gene, responsible for the synthesis of a substrate break enzyme, described in Pelomonas sacchraphyla (sucrose phosphorylase) were utilized. The genetic engineering proposed in this study is innovative, and has never been done before. The production of rhamnolipid in large-scale at low costs will be highly beneficial for the development of national technologies for production of high value-added chemical.Biossurfactantes s??o metab??litos produzidos por diversos microrganismos. Estes cont??m um dom??nio hidrof??lico e outro hidrof??bico, estando preferencialmente na interface de compostos polares e apolares. Devido ??s suas propriedades, os biosurfactantes s??o capazes de reduzir a tens??o de superf??cies tendo uma ampla aplica????o na ind??stria farmac??utica, cosm??tica, aliment??cia e agr??cola. O uso de biossurfactante em compara????o com os de origem qu??mica s??o considerados vantajosos, uma vez que s??o biodegrad??veis e n??o t??xicos. Atualmente um dos biossurfactantes mais conhecido e dispon??vel comercialmente s??o os ramnolip??deos. No entanto, a utiliza????o destas mol??culas em larga escala ?? prejudicada devido aos seus elevados custos de produ????o. Ramnolip??deos s??o produzidos principalmente por Pseudomonas aeruginosa e s??o constitu??dos por uma ou duas mol??culas de L-ramnose ligada a uma mol??cula de ??cido graxo. Assim, este estudo teve como objetivo a constru????o de vetores de express??o em levedura para posterior constru????o de cepas recombinantes de Saccharomyces cerevisiae visando a eficiente produ????o de L-ramnose e monoramnolip??deo a partir de sacarose. Para a constru????o desses vetores, foram utilizados seis genes, que codificam as enzimas respons??veis pelas vias de bioss??ntese de Lramnose e mono-ramnolipideos descritos em P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA e rhlB), e um gene, respons??vel pela s??ntese da enzima de quebra do substrato, descrito em Pelomonas sacchraphyla (sacarose fosforilase). A engenharia gen??tica proposta neste estudo ?? inovadora, e pela primeira vez realizada. Com a produ????o do ramnolip??deo em larga escala ?? um baixo custo de produ????o, beneficiar?? o desenvolvimento de tecnologias nacionais para produ????o de qu??micos de alto valor agregado.Submitted by Kelson Anthony de Menezes (kelson@ucb.br) on 2016-12-19T18:06:48Z No. of bitstreams: 1 RayaneLuziaViegasCamposDissertacao2015.pdf: 2888012 bytes, checksum: 8f9962ac7690fc08e072b81ccd8516db (MD5)Made available in DSpace on 2016-12-19T18:06:48Z (GMT). No. of bitstreams: 1 RayaneLuziaViegasCamposDissertacao2015.pdf: 2888012 bytes, checksum: 8f9962ac7690fc08e072b81ccd8516db (MD5) Previous issue date: 2015-03-31application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/4078/RayaneLuziaViegasCamposDissertacao2015.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma Strictu Sensu em Ci??ncias Gen??micas e BiotecnologiaUCBBrasilEscola de Sa??de e MedicinaBiossurfactanteRamnolip??deoProdu????oLarga escalaSaccharomyces cerevisiaeGen??ticaGenomaCIENCIAS BIOLOGICAS::GENETICASuperprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??oinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-2827197273900952156500500600-6392058866414562720-5518144268585252051info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasíliainstacron:UCBTHUMBNAILRayaneLuziaViegasCamposDissertacao2015.pdf.jpgRayaneLuziaViegasCamposDissertacao2015.pdf.jpgimage/jpeg5672https://bdtd.ucb.br:8443/jspui/bitstream/tede/1997/3/RayaneLuziaViegasCamposDissertacao2015.pdf.jpg4e21003ecb6a4859e04aef76e31635c1MD53ORIGINALRayaneLuziaViegasCamposDissertacao2015.pdfRayaneLuziaViegasCamposDissertacao2015.pdfapplication/pdf2888012https://bdtd.ucb.br:8443/jspui/bitstream/tede/1997/2/RayaneLuziaViegasCamposDissertacao2015.pdf8f9962ac7690fc08e072b81ccd8516dbMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089https://bdtd.ucb.br:8443/jspui/bitstream/tede/1997/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51tede/1997oai:bdtd.ucb.br:tede/19972016-12-20 01:02:36.375Biblioteca Digital de Disserta????es da Universidade Cat??lica de Bras??lia - UCBsdi@ucb.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
dc.title.por.fl_str_mv Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
title Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
spellingShingle Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
Campos, Rayane Luzia Viegas Campos
Biossurfactante
Ramnolip??deo
Produ????o
Larga escala
Saccharomyces cerevisiae
Gen??tica
Genoma
CIENCIAS BIOLOGICAS::GENETICA
title_short Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
title_full Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
title_fullStr Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
title_full_unstemmed Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
title_sort Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o
author Campos, Rayane Luzia Viegas Campos
author_facet Campos, Rayane Luzia Viegas Campos
author_role author
dc.contributor.advisor1.fl_str_mv Parachin, N??dia Skorupa
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1214716137780644
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3454004547074775
dc.contributor.author.fl_str_mv Campos, Rayane Luzia Viegas Campos
contributor_str_mv Parachin, N??dia Skorupa
dc.subject.por.fl_str_mv Biossurfactante
Ramnolip??deo
Produ????o
Larga escala
Saccharomyces cerevisiae
Gen??tica
Genoma
topic Biossurfactante
Ramnolip??deo
Produ????o
Larga escala
Saccharomyces cerevisiae
Gen??tica
Genoma
CIENCIAS BIOLOGICAS::GENETICA
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::GENETICA
dc.description.abstract.eng.fl_txt_mv Biosurfactants are metabolites that contain both hydrophobic and hydrophilic domains and are produced by various microorganisms. Due to its characteristics such molecules localize preferable in the interface of polar and non-polar solvents. Biosurfactants are widely used in pharmaceutic, cosmetic, food and agriculture industries. Moreover, they are considered advantageous when compared to chemical ones because they are biodegradable and non-toxic. Currently one of the best known biosurfactant is the rhamnolipids. However, their utilization in large scale are still not possible due to its high production costs. Rhamnolipids are produced mainly by Pseudomonas aeruginosa and are composed by one or two molecules of L-rhamnose attached to a fatty acid molecule. This study aimed the construction of yeast expression vectors that will be used to construct a recombinant strain of Saccharomyces cerevisiae efficient in the production of L-rhamnose and mono-rhamnolipid using sucrose as substrate. For the construction of these vectors, six different genes, responsible for encoding both L-rhamnose and mono-ramnolipideos biosynthetic pathways enzymes described in P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA and rhlB), and one gene, responsible for the synthesis of a substrate break enzyme, described in Pelomonas sacchraphyla (sucrose phosphorylase) were utilized. The genetic engineering proposed in this study is innovative, and has never been done before. The production of rhamnolipid in large-scale at low costs will be highly beneficial for the development of national technologies for production of high value-added chemical.
dc.description.abstract.por.fl_txt_mv Biossurfactantes s??o metab??litos produzidos por diversos microrganismos. Estes cont??m um dom??nio hidrof??lico e outro hidrof??bico, estando preferencialmente na interface de compostos polares e apolares. Devido ??s suas propriedades, os biosurfactantes s??o capazes de reduzir a tens??o de superf??cies tendo uma ampla aplica????o na ind??stria farmac??utica, cosm??tica, aliment??cia e agr??cola. O uso de biossurfactante em compara????o com os de origem qu??mica s??o considerados vantajosos, uma vez que s??o biodegrad??veis e n??o t??xicos. Atualmente um dos biossurfactantes mais conhecido e dispon??vel comercialmente s??o os ramnolip??deos. No entanto, a utiliza????o destas mol??culas em larga escala ?? prejudicada devido aos seus elevados custos de produ????o. Ramnolip??deos s??o produzidos principalmente por Pseudomonas aeruginosa e s??o constitu??dos por uma ou duas mol??culas de L-ramnose ligada a uma mol??cula de ??cido graxo. Assim, este estudo teve como objetivo a constru????o de vetores de express??o em levedura para posterior constru????o de cepas recombinantes de Saccharomyces cerevisiae visando a eficiente produ????o de L-ramnose e monoramnolip??deo a partir de sacarose. Para a constru????o desses vetores, foram utilizados seis genes, que codificam as enzimas respons??veis pelas vias de bioss??ntese de Lramnose e mono-ramnolipideos descritos em P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA e rhlB), e um gene, respons??vel pela s??ntese da enzima de quebra do substrato, descrito em Pelomonas sacchraphyla (sacarose fosforilase). A engenharia gen??tica proposta neste estudo ?? inovadora, e pela primeira vez realizada. Com a produ????o do ramnolip??deo em larga escala ?? um baixo custo de produ????o, beneficiar?? o desenvolvimento de tecnologias nacionais para produ????o de qu??micos de alto valor agregado.
description Biosurfactants are metabolites that contain both hydrophobic and hydrophilic domains and are produced by various microorganisms. Due to its characteristics such molecules localize preferable in the interface of polar and non-polar solvents. Biosurfactants are widely used in pharmaceutic, cosmetic, food and agriculture industries. Moreover, they are considered advantageous when compared to chemical ones because they are biodegradable and non-toxic. Currently one of the best known biosurfactant is the rhamnolipids. However, their utilization in large scale are still not possible due to its high production costs. Rhamnolipids are produced mainly by Pseudomonas aeruginosa and are composed by one or two molecules of L-rhamnose attached to a fatty acid molecule. This study aimed the construction of yeast expression vectors that will be used to construct a recombinant strain of Saccharomyces cerevisiae efficient in the production of L-rhamnose and mono-rhamnolipid using sucrose as substrate. For the construction of these vectors, six different genes, responsible for encoding both L-rhamnose and mono-ramnolipideos biosynthetic pathways enzymes described in P. aeruginosa (rmlA, rmlB, rmlC, rmlD, rhlA and rhlB), and one gene, responsible for the synthesis of a substrate break enzyme, described in Pelomonas sacchraphyla (sucrose phosphorylase) were utilized. The genetic engineering proposed in this study is innovative, and has never been done before. The production of rhamnolipid in large-scale at low costs will be highly beneficial for the development of national technologies for production of high value-added chemical.
publishDate 2015
dc.date.issued.fl_str_mv 2015-03-31
dc.date.accessioned.fl_str_mv 2016-12-19T18:06:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
format masterThesis
dc.identifier.citation.fl_str_mv CAMPOS, Rayane Luzia Viegas Campos. Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o. 2015. 65f. Disserta????o( Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2015.
dc.identifier.uri.fl_str_mv https://bdtd.ucb.br:8443/jspui/handle/tede/1997
identifier_str_mv CAMPOS, Rayane Luzia Viegas Campos. Superprodu????o de ramnolip??deo em Saccharomyces cerevisiae: Constru????o de vetores de express??o. 2015. 65f. Disserta????o( Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2015.
url https://bdtd.ucb.br:8443/jspui/handle/tede/1997
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dc.publisher.program.fl_str_mv Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia
dc.publisher.initials.fl_str_mv UCB
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Sa??de e Medicina
publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
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