Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Silva, Nara Cristina lattes
Orientador(a): Araújo, Eugênio Gonçalves de lattes
Banca de defesa: Pôrto, Regiani Nascimento Gagno, Santin, Ana Paula Nascimento Iglesias, Araújo, Eugênio Gonçalves de
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Goiás
Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
País: Brasil
Palavras-chave em Português:
Neoplasia óssea
Cultivo celular
MG-63
Palavras-chave em Inglês:
Bone neoplasms
Cell culture
Área do conhecimento CNPq:
CIENCIAS AGRARIAS::ZOOTECNIA
Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/9185
Resumo: Osteosarcoma is a highly aggressive malignant bone tumor that has an unfavorable prognosis. The treatment of choice is surgical intervention associated with systemic chemotherapy. However, the chemotherapeutic agents currently employed have several adverse effects on patients that limit their use. Thus, research aimed at new alternatives to the treatment of this cancer is of paramount importance. This study aimed to evaluate, in vitro, the cytotoxic potential of extracts from leaves of gabirobeira in osteosarcoma cells. From the leaves of this plant, the crude ethanolic extract and ethyl acetate were extracted. The cells were cultured and treated with both extracts at different concentrations and at predetermined exposure times. Cell cytotoxicity was analyzed by the Tripan blue exclusion assay and the IC50 calculated by the tetrazolium reduction method. The crude extract presented higher cytotoxicity compared to acetate, and the best result was in the 1.0μL/mL group treated for 48 hours. In addition, the IC50 value for G48 is within the range suggested as the best cytotoxicity. Finally, extracts of the leaves of the gabirobeira presented cytotoxic action in the cells of in vitro osteosarcoma.
id UFG-2_8c912f8de45f3533cf0f2b2b2fea95ef
oai_identifier_str oai:repositorio.bc.ufg.br:tede/9185
network_acronym_str UFG-2
network_name_str Repositório Institucional da UFG
repository_id_str
spelling Araújo, Eugênio Gonçalves dehttp://lattes.cnpq.br/3919777570059928Cruz, Vanessa de Sousahttp://lattes.cnpq.br/8788967925940484Damasceno, Adilson Donizetihttp://lattes.cnpq.br/3900110295277130Pôrto, Regiani Nascimento GagnoSantin, Ana Paula Nascimento IglesiasAraújo, Eugênio Gonçalves dehttp://lattes.cnpq.br/5309447416219108Silva, Nara Cristina2018-12-26T11:33:36Z2018-06-29SILVA, N. C. Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro. 2018. 52 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/9185Osteosarcoma is a highly aggressive malignant bone tumor that has an unfavorable prognosis. The treatment of choice is surgical intervention associated with systemic chemotherapy. However, the chemotherapeutic agents currently employed have several adverse effects on patients that limit their use. Thus, research aimed at new alternatives to the treatment of this cancer is of paramount importance. This study aimed to evaluate, in vitro, the cytotoxic potential of extracts from leaves of gabirobeira in osteosarcoma cells. From the leaves of this plant, the crude ethanolic extract and ethyl acetate were extracted. The cells were cultured and treated with both extracts at different concentrations and at predetermined exposure times. Cell cytotoxicity was analyzed by the Tripan blue exclusion assay and the IC50 calculated by the tetrazolium reduction method. The crude extract presented higher cytotoxicity compared to acetate, and the best result was in the 1.0μL/mL group treated for 48 hours. In addition, the IC50 value for G48 is within the range suggested as the best cytotoxicity. Finally, extracts of the leaves of the gabirobeira presented cytotoxic action in the cells of in vitro osteosarcoma.O osteossarcoma é um tumor ósseo maligno, altamente agressivo que possui prognóstico desfavorável. O tratamento de escolha é a intervenção cirúrgica associada à quimioterapia sistêmica. Entretanto, os quimioterápicos empregados atualmente possuem diversos efeitos adversos aos pacientes que limitam o seu uso. Assim, pesquisas que visam novas alternativas ao tratamento desse câncer são de suma importância. Com este estudo, objetivou-se avaliar, in vitro, o potencial citotóxico de extratos das folhas da gabirobeira em células de osteossarcoma. A partir das folhas dessa planta, foi extraído o extrato etanólico bruto e acetato de etila. As células foram cultivadas e tratadas com ambos os extratos em diferentes concentrações e tempos de exposição pré-determinados. Foi analisada a citotoxicidade celular por meio do ensaio de exclusão do azul de Tripan e calculado o IC50 pelo método de redução dotetrazólio. O extrato bruto apresentou maior citotoxicidade em comparação com o acetato, sendo o melhor resultado no grupo tratado com 1,0μL/mL durante 48 horas. Ademais, o valor do IC50 para o G48 situa-se no intervalo sugerido como melhor citotoxicidade. Por fim, os extratos das folhas da gabirobeira apresentaram ação citotóxica nas células de osteossarcoma in vitro.application/pdfporUniversidade Federal de GoiásPrograma de Pós-graduação em Ciência Animal (EVZ)UFGBrasilEscola de Veterinária e Zootecnia - EVZ (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessNeoplasia ósseaCultivo celularMG-63Bone neoplasmsCell cultureCIENCIAS AGRARIAS::ZOOTECNIACitotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis4581960685150189167600600600-62175521142490945821346858981270845602reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/493d69d1-81fe-478b-ba19-047406fc0ebd/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/3f7bdde3-0c03-42cd-90ba-c4eb8a9fdc46/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/e26138d0-74a7-4121-8ec1-0f1f35d1ee70/downloadd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.bc.ufg.br/tede/bitstreams/5e7f33f0-bcae-4553-8a9c-d4a6d177cab2/downloadd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALDissertação - Nara Cristina Silva - 2018.pdfDissertação - Nara Cristina Silva - 2018.pdfapplication/pdf3522672http://repositorio.bc.ufg.br/tede/bitstreams/7f1a3f9c-8449-440e-88eb-a3e7955b2389/download86242f639764339f703e447cee75aca4MD55tede/91852018-12-26 09:33:37.036http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/9185http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttps://repositorio.bc.ufg.br/tedeserver/oai/requestgrt.bc@ufg.bropendoar:oai:repositorio.bc.ufg.br:tede/12342018-12-26T11:33:37Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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
dc.title.eng.fl_str_mv Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
title Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
spellingShingle Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
Silva, Nara Cristina
Neoplasia óssea
Cultivo celular
MG-63
Bone neoplasms
Cell culture
CIENCIAS AGRARIAS::ZOOTECNIA
title_short Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
title_full Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
title_fullStr Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
title_full_unstemmed Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
title_sort Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro
author Silva, Nara Cristina
author_facet Silva, Nara Cristina
author_role author
dc.contributor.advisor1.fl_str_mv Araújo, Eugênio Gonçalves de
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3919777570059928
dc.contributor.advisor-co1.fl_str_mv Cruz, Vanessa de Sousa
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/8788967925940484
dc.contributor.advisor-co2.fl_str_mv Damasceno, Adilson Donizeti
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/3900110295277130
dc.contributor.referee1.fl_str_mv Pôrto, Regiani Nascimento Gagno
dc.contributor.referee2.fl_str_mv Santin, Ana Paula Nascimento Iglesias
dc.contributor.referee3.fl_str_mv Araújo, Eugênio Gonçalves de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5309447416219108
dc.contributor.author.fl_str_mv Silva, Nara Cristina
contributor_str_mv Araújo, Eugênio Gonçalves de
Cruz, Vanessa de Sousa
Damasceno, Adilson Donizeti
Pôrto, Regiani Nascimento Gagno
Santin, Ana Paula Nascimento Iglesias
Araújo, Eugênio Gonçalves de
dc.subject.por.fl_str_mv Neoplasia óssea
Cultivo celular
MG-63
topic Neoplasia óssea
Cultivo celular
MG-63
Bone neoplasms
Cell culture
CIENCIAS AGRARIAS::ZOOTECNIA
dc.subject.eng.fl_str_mv Bone neoplasms
Cell culture
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::ZOOTECNIA
description Osteosarcoma is a highly aggressive malignant bone tumor that has an unfavorable prognosis. The treatment of choice is surgical intervention associated with systemic chemotherapy. However, the chemotherapeutic agents currently employed have several adverse effects on patients that limit their use. Thus, research aimed at new alternatives to the treatment of this cancer is of paramount importance. This study aimed to evaluate, in vitro, the cytotoxic potential of extracts from leaves of gabirobeira in osteosarcoma cells. From the leaves of this plant, the crude ethanolic extract and ethyl acetate were extracted. The cells were cultured and treated with both extracts at different concentrations and at predetermined exposure times. Cell cytotoxicity was analyzed by the Tripan blue exclusion assay and the IC50 calculated by the tetrazolium reduction method. The crude extract presented higher cytotoxicity compared to acetate, and the best result was in the 1.0μL/mL group treated for 48 hours. In addition, the IC50 value for G48 is within the range suggested as the best cytotoxicity. Finally, extracts of the leaves of the gabirobeira presented cytotoxic action in the cells of in vitro osteosarcoma.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-12-26T11:33:36Z
dc.date.issued.fl_str_mv 2018-06-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, N. C. Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro. 2018. 52 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/9185
identifier_str_mv SILVA, N. C. Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro. 2018. 52 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.
url http://repositorio.bc.ufg.br/tede/handle/tede/9185
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 4581960685150189167
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv -6217552114249094582
dc.relation.cnpq.fl_str_mv 1346858981270845602
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Ciência Animal (EVZ)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Veterinária e Zootecnia - EVZ (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Repositório Institucional da UFG
collection Repositório Institucional da UFG
bitstream.url.fl_str_mv http://repositorio.bc.ufg.br/tede/bitstreams/493d69d1-81fe-478b-ba19-047406fc0ebd/download
http://repositorio.bc.ufg.br/tede/bitstreams/3f7bdde3-0c03-42cd-90ba-c4eb8a9fdc46/download
http://repositorio.bc.ufg.br/tede/bitstreams/e26138d0-74a7-4121-8ec1-0f1f35d1ee70/download
http://repositorio.bc.ufg.br/tede/bitstreams/5e7f33f0-bcae-4553-8a9c-d4a6d177cab2/download
http://repositorio.bc.ufg.br/tede/bitstreams/7f1a3f9c-8449-440e-88eb-a3e7955b2389/download
bitstream.checksum.fl_str_mv bd3efa91386c1718a7f26a329fdcb468
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
86242f639764339f703e447cee75aca4
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv grt.bc@ufg.br
_version_ 1861293783043276800

Registros relacionados