Propriedades citotóxicas da Beta-Lapachona em células de osteossarcoma in vitro

• Ano de defesa: 2017
• Autor(a) principal: Gabriel, Gabriela Hadler
• Orientador(a): Araújo, Eugênio Gonçalves de
• Banca de defesa: Araújo, Eugênio Gonçalves de , Oliveira, Cairo Henrique Sousa de, Cruz, Vanessa de Sousa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Ciência Animal (EVZ)
• Departamento: Escola de Veterinária e Zootecnia - EVZ (RG)
• País: Brasil
• Palavras-chave em Português: Cultivo celular; Linhagem MG-63; Naftoquinona; Neoplasia óssea
• Palavras-chave em Inglês: Bone tumor; Cell culture; MG-63 cell line; Naphthoquinone
• Área do conhecimento CNPq: MEDICINA VETERINARIA::PATOLOGIA ANIMAL
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/7150

Osteosarcoma is the main primary bone tumor, with unfavorable prognosis, high mortality and high incidence of metastases. The treatment of choice is the removal of the tumor associated with combined chemotherapy, whose adverse effects allude to the increasing need to develop new drugs. The plants constitute a large natural reserve of compounds that have medicinal and therapeutic properties, such as lapachol and its derivative, β-lapachone. The aim of this study was to evaluate the cytotoxic effects of β-lapachone in osteosarcoma cells cultured in vitro. Cells were cultured and treated with β-lapachone at different concentrations and times of exposure. Tripan blue exclusion, tetrazolium reduction and cell survival assay methods were performed to evaluate the effects of the compound on the cells. Cells treated with 0,1μM β-lapachone showed lower initial cytotoxicity in the 24h time, whereas those submitted to 1,0μM showed less viability after 72h of treatment. Cytotoxicity increased as the concentration and time of exposure increased. The lowest IC50 (0,148μM) was observed in treated cells for 72h. Cell growth after treatment was lower in the 1.0μl group after 72h and the highest cell growth was observed under a concentration of 0.1μl after 24h. There was no difference between groups for cell proliferation after treatment, and the cell survival fraction was lower after 72h of exposure. It was concluded that β-lapachone has cytotoxic effects on osteosarcoma cells cultured in vitro.