Obtenção de dispersões sólidas contendo naringenina e avaliação in vitro, in vivo e ex vivo da modulação das enzimas do sistema colinérgico

• Ano de defesa: 2022
• Autor(a) principal: Cunha, Viviane Augusta de Medeiros Garcia
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Tecnológica Federal do Paraná; Campo Mourao; Medianeira; Brasil; Programa de Pós-Graduação em Tecnologia de Alimentos; UTFPR
• Palavras-chave em Português: Nanopartículas; Antioxidantes; Cinética enzimática; Colina (Química); Nanoparticles; Antioxidants; Enzyme Kinetics; Choline; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS; Tecnologia de Alimentos
• Link de acesso: http://repositorio.utfpr.edu.br/jspui/handle/1/29738

Naringenin is a flavonoid derived from naringin, being considered a glycated polyphenol of the flavanone class present in citrus plants such as grapefruit (Citrus paradisi). It has antioxidant properties, and recent studies suggest its effectiveness in treating pathologies related to the central nervous system such as cerebral ischemia. The reversible inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activity resulting from the action of naringenin reveals its potential for the treatment of diseases of the nervous system, such as Alzheimer's disease. Its low affinity for water makes its use in aqueous foods a technological challenge and methods for obtaining solid dispersions have been shown to be effective in overcoming such negative aspects of naringenin. However, it is necessary to assess how encapsulation can affect naringenin activity. The present work evaluates the action of naringenin and solid dispersions containing naringenin on the enzymes of the cholinergic system in in vitro, in vivo and ex vivo assays. The solid dispersion was characterized by Differential Scanning Calorimetry (DSC) and by Forier Transform Infrared Spectroscopy (FTIR). In vitro assays analyzed the activity and kinetics of AChE and BChE enzymes using the modified Ellman method. In vivo analyzes were performed on Drosophila melanogaster (DM) performing the survival rate test and ex vivo analysis of AChE and BChE enzyme activity using the modified Ellman method, in addition to catalase and superoxide dismutase enzyme activities. The solid dispersion characterization results suggest that the nanoencapsulation of naringenin occurred satisfactorily. The in vitro assays performed showed that there was a noncompetitive inhibition of naringenin in relation to the AChE and BChE enzymes, presenting IC50 values for the AChE enzyme of 99.4 µM and for the BChE of 627.6 µM. The survival rate assay showed that both free naringenin and solid dispersion did not change the lifespan of DM, indicating low toxicity. The ex vivo DM assays against AChE and BChE enzymes did not show naringenin inhibition activity, however the analyzes with catalase and superoxide dismutase confirmed the antioxidant activity of naringenin also found in the literature. Therefore, the results obtained from the characterization of the solid dispersion technique in the production of nanoparticles containing naringenin showed the efficiency of encapsulation as well as the results of AChE and BChE enzymes against free naringenin confirmed the inhibition of these enzymes. Naringenin then presented results that corroborate the literature and contribute to future research for its use in foods.